<STUDY_REF refname="phs000228_31" refcenter="NHGRI" accession="SRP002860"> <IDENTIFIERS> <PRIMARY_ID>SRP002860</PRIMARY_ID> <SUBMITTER_ID namespace="NHGRI">phs000228_31</SUBMITTER_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>LS454 sequencing of Human Microbiome Project 16S rRNA 454 Clinical Production Phase II 2855015837 fragment PCR library</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR refcenter="NCBI" accession="SRS011687"> <POOL> <DEFAULT_MEMBER> <IDENTIFIERS> <PRIMARY_ID>SRS011687</PRIMARY_ID> </IDENTIFIERS> </DEFAULT_MEMBER> <MEMBER member_name="2855230053_700103585_V1-V3" refname="700103585" refcenter="NCBI" accession="SRS043664"> <IDENTIFIERS> <PRIMARY_ID>SRS043664</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103585</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="ATACCAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230074_700105870_V1-V3" refname="700105870" refcenter="NCBI" accession="SRS055253"> <IDENTIFIERS> <PRIMARY_ID>SRS055253</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105870</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CGGTATC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230060_700105600_V3-V5" refname="700105600" refcenter="NCBI" accession="SRS054796"> <IDENTIFIERS> <PRIMARY_ID>SRS054796</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105600</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="AGTTGGC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230068_700105455_V3-V5" refname="700105455" refcenter="NCBI" accession="SRS051166"> <IDENTIFIERS> <PRIMARY_ID>SRS051166</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105455</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CTTCCTTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230080_700105872_V3-V5" refname="700105872" refcenter="NCBI" accession="SRS053092"> <IDENTIFIERS> <PRIMARY_ID>SRS053092</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105872</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TGACGAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230138_700105433_V1-V3" refname="700105433" refcenter="NCBI" accession="SRS044643"> <IDENTIFIERS> <PRIMARY_ID>SRS044643</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105433</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AAGGCAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230077_700105425_V3-V5" refname="700105425" refcenter="NCBI" accession="SRS055952"> <IDENTIFIERS> <PRIMARY_ID>SRS055952</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105425</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="ACTCCTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230061_700105230_V3-V5" refname="700105230" refcenter="NCBI" accession="SRS042523"> <IDENTIFIERS> <PRIMARY_ID>SRS042523</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105230</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="CCTTCCGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230072_700105447_V3-V5" refname="700105447" refcenter="NCBI" accession="SRS056523"> <IDENTIFIERS> <PRIMARY_ID>SRS056523</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105447</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TGCCGAAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230076_700106665_V3-V5" refname="700106665" refcenter="NCBI" accession="SRS056795"> <IDENTIFIERS> <PRIMARY_ID>SRS056795</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106665</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="CACGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230081_700106679_V1-V3" refname="700106679" refcenter="NCBI" accession="SRS054954"> <IDENTIFIERS> <PRIMARY_ID>SRS054954</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106679</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CGTGAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230051_700105715_V3-V5" refname="700105715" refcenter="NCBI" accession="SRS054477"> <IDENTIFIERS> <PRIMARY_ID>SRS054477</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105715</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TCTCGAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230050_700114068_V3-V5" refname="700114068" refcenter="NCBI" accession="SRS063070"> <IDENTIFIERS> <PRIMARY_ID>SRS063070</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700114068</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TGACCGTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230141_700106362_V3-V5" refname="700106362" refcenter="NCBI" accession="SRS050055"> <IDENTIFIERS> <PRIMARY_ID>SRS050055</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106362</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AGGCGGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230143_700103637_V3-V5" refname="700103637" refcenter="NCBI" accession="SRS044526"> <IDENTIFIERS> <PRIMARY_ID>SRS044526</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103637</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TTAAGATTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230140_700103448_V3-V5" refname="700103448" refcenter="NCBI" accession="SRS048692"> <IDENTIFIERS> <PRIMARY_ID>SRS048692</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103448</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AGGTTGTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230075_700106633_V1-V3" refname="700106633" refcenter="NCBI" accession="SRS045165"> <IDENTIFIERS> <PRIMARY_ID>SRS045165</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106633</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CGGTCTTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230073_700105604_V1-V3" refname="700105604" refcenter="NCBI" accession="SRS058326"> <IDENTIFIERS> <PRIMARY_ID>SRS058326</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105604</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="AACCTGGC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230057_700105769_V3-V5" refname="700105769" refcenter="NCBI" accession="SRS044569"> <IDENTIFIERS> <PRIMARY_ID>SRS044569</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105769</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TCACCTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230136_700106645_V1-V3" refname="700106645" refcenter="NCBI" accession="SRS055669"> <IDENTIFIERS> <PRIMARY_ID>SRS055669</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106645</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="ACTAATTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230058_700105858_V3-V5" refname="700105858" refcenter="NCBI" accession="SRS049858"> <IDENTIFIERS> <PRIMARY_ID>SRS049858</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105858</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CAAGAAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230055_700114023_V1-V3" refname="700114023" refcenter="NCBI" accession="SRS063248"> <IDENTIFIERS> <PRIMARY_ID>SRS063248</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700114023</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TGAAGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230054_700105741_V1-V3" refname="700105741" refcenter="NCBI" accession="SRS058170"> <IDENTIFIERS> <PRIMARY_ID>SRS058170</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105741</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AGACCTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230065_700106693_V1-V3" refname="700106693" refcenter="NCBI" accession="SRS049575"> <IDENTIFIERS> <PRIMARY_ID>SRS049575</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106693</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="ACTCAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230070_700105592_V1-V3" refname="700105592" refcenter="NCBI" accession="SRS051269"> <IDENTIFIERS> <PRIMARY_ID>SRS051269</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105592</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="AGCTTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230059_700105598_V1-V3" refname="700105598" refcenter="NCBI" accession="SRS051929"> <IDENTIFIERS> <PRIMARY_ID>SRS051929</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105598</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CGACTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230067_700105254_V3-V5" refname="700105254" refcenter="NCBI" accession="SRS045212"> <IDENTIFIERS> <PRIMARY_ID>SRS045212</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105254</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="ACCTTGGTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230064_700103452_V1-V3" refname="700103452" refcenter="NCBI" accession="SRS057461"> <IDENTIFIERS> <PRIMARY_ID>SRS057461</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103452</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="ACGAGAAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230134_700103243_V3-V5" refname="700103243" refcenter="NCBI" accession="SRS053264"> <IDENTIFIERS> <PRIMARY_ID>SRS053264</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103243</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TGCGTTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230052_700106906_V3-V5" refname="700106906" refcenter="NCBI" accession="SRS042558"> <IDENTIFIERS> <PRIMARY_ID>SRS042558</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106906</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="CCTCTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230048_700105648_V1-V3" refname="700105648" refcenter="NCBI" accession="SRS051098"> <IDENTIFIERS> <PRIMARY_ID>SRS051098</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105648</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="ACACGGAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230135_700114141_V1-V3" refname="700114141" refcenter="NCBI" accession="SRS063236"> <IDENTIFIERS> <PRIMARY_ID>SRS063236</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700114141</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TTGACAAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230062_700105590_V3-V5" refname="700105590" refcenter="NCBI" accession="SRS043369"> <IDENTIFIERS> <PRIMARY_ID>SRS043369</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105590</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TATCAAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230082_700105284_V3-V5" refname="700105284" refcenter="NCBI" accession="SRS055631"> <IDENTIFIERS> <PRIMARY_ID>SRS055631</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105284</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AGACAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230049_700105992_V3-V5" refname="700105992" refcenter="NCBI" accession="SRS042788"> <IDENTIFIERS> <PRIMARY_ID>SRS042788</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105992</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TTCGAGC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230069_700105656_V3-V5" refname="700105656" refcenter="NCBI" accession="SRS046281"> <IDENTIFIERS> <PRIMARY_ID>SRS046281</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105656</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CGTCGTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230056_700103623_V1-V3" refname="700103623" refcenter="NCBI" accession="SRS051029"> <IDENTIFIERS> <PRIMARY_ID>SRS051029</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103623</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TCGAGGAAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230063_700103456_V3-V5" refname="700103456" refcenter="NCBI" accession="SRS047354"> <IDENTIFIERS> <PRIMARY_ID>SRS047354</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103456</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TCGTTGTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230142_700103462_V3-V5" refname="700103462" refcenter="NCBI" accession="SRS058246"> <IDENTIFIERS> <PRIMARY_ID>SRS058246</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103462</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TTGGAGGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230071_700105606_V1-V3" refname="700105606" refcenter="NCBI" accession="SRS056058"> <IDENTIFIERS> <PRIMARY_ID>SRS056058</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105606</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="ACGAAGTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230066_700103710_V3-V5" refname="700103710" refcenter="NCBI" accession="SRS043411"> <IDENTIFIERS> <PRIMARY_ID>SRS043411</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103710</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TTCGTGGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230139_700103587_V1-V3" refname="700103587" refcenter="NCBI" accession="SRS044606"> <IDENTIFIERS> <PRIMARY_ID>SRS044606</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103587</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="AAGCCGC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230137_700103702_V3-V5" refname="700103702" refcenter="NCBI" accession="SRS056473"> <IDENTIFIERS> <PRIMARY_ID>SRS056473</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103702</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AATGGTAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230083_700105642_V3-V5" refname="700105642" refcenter="NCBI" accession="SRS058322"> <IDENTIFIERS> <PRIMARY_ID>SRS058322</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105642</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TAGGAATC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> </POOL> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>2855015837</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <SINGLE/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> <SPOT_DESCRIPTOR> <SPOT_DECODE_SPEC> <READ_SPEC> <READ_INDEX>0</READ_INDEX> <READ_CLASS>Technical Read</READ_CLASS> <READ_TYPE>Adapter</READ_TYPE> <BASE_COORD>1</BASE_COORD> </READ_SPEC> <READ_SPEC> <READ_INDEX>1</READ_INDEX> <READ_LABEL>barcode</READ_LABEL> <READ_CLASS>Technical Read</READ_CLASS> <READ_TYPE>BarCode</READ_TYPE> <EXPECTED_BASECALL_TABLE> <BASECALL read_group_tag="AACCTGGC" min_match="8" max_mismatch="0" match_edge="full">AACCTGGC</BASECALL> <BASECALL read_group_tag="CGTCGTC" min_match="7" max_mismatch="0" match_edge="full">CGTCGTC</BASECALL> <BASECALL read_group_tag="TGCCGAAC" min_match="8" max_mismatch="0" match_edge="full">TGCCGAAC</BASECALL> <BASECALL read_group_tag="CGGTCTTC" min_match="8" max_mismatch="0" match_edge="full">CGGTCTTC</BASECALL> <BASECALL read_group_tag="ACTAATTC" min_match="8" max_mismatch="0" match_edge="full">ACTAATTC</BASECALL> <BASECALL read_group_tag="AAGGCAC" min_match="7" max_mismatch="0" match_edge="full">AAGGCAC</BASECALL> <BASECALL read_group_tag="TGACCGTC" min_match="8" max_mismatch="0" match_edge="full">TGACCGTC</BASECALL> <BASECALL read_group_tag="TTGGAGGC" min_match="8" max_mismatch="0" match_edge="full">TTGGAGGC</BASECALL> <BASECALL read_group_tag="ACGAGAAC" min_match="8" max_mismatch="0" match_edge="full">ACGAGAAC</BASECALL> <BASECALL read_group_tag="TCACCTC" min_match="7" max_mismatch="0" match_edge="full">TCACCTC</BASECALL> <BASECALL read_group_tag="AGGCGGC" min_match="7" max_mismatch="0" match_edge="full">AGGCGGC</BASECALL> <BASECALL read_group_tag="AGGTTGTC" min_match="8" max_mismatch="0" match_edge="full">AGGTTGTC</BASECALL> <BASECALL read_group_tag="CGGTATC" min_match="7" max_mismatch="0" match_edge="full">CGGTATC</BASECALL> <BASECALL read_group_tag="TATCAAC" min_match="7" max_mismatch="0" match_edge="full">TATCAAC</BASECALL> <BASECALL read_group_tag="CTTCCTTC" min_match="8" max_mismatch="0" match_edge="full">CTTCCTTC</BASECALL> <BASECALL read_group_tag="CCTCTC" min_match="6" max_mismatch="0" match_edge="full">CCTCTC</BASECALL> <BASECALL read_group_tag="TTCGAGC" min_match="7" max_mismatch="0" match_edge="full">TTCGAGC</BASECALL> <BASECALL read_group_tag="AGTTGGC" min_match="7" max_mismatch="0" match_edge="full">AGTTGGC</BASECALL> <BASECALL read_group_tag="CGACTC" min_match="6" max_mismatch="0" match_edge="full">CGACTC</BASECALL> <BASECALL read_group_tag="AGCTTC" min_match="6" max_mismatch="0" match_edge="full">AGCTTC</BASECALL> <BASECALL read_group_tag="CACGC" min_match="5" max_mismatch="0" match_edge="full">CACGC</BASECALL> <BASECALL read_group_tag="AATGGTAC" min_match="8" max_mismatch="0" match_edge="full">AATGGTAC</BASECALL> <BASECALL read_group_tag="AAGCCGC" min_match="7" max_mismatch="0" match_edge="full">AAGCCGC</BASECALL> <BASECALL read_group_tag="TCTCGAC" min_match="7" max_mismatch="0" match_edge="full">TCTCGAC</BASECALL> <BASECALL read_group_tag="TGAAGC" min_match="6" max_mismatch="0" match_edge="full">TGAAGC</BASECALL> <BASECALL read_group_tag="CAAGAAC" min_match="7" max_mismatch="0" match_edge="full">CAAGAAC</BASECALL> <BASECALL read_group_tag="TTCGTGGC" min_match="8" max_mismatch="0" match_edge="full">TTCGTGGC</BASECALL> <BASECALL read_group_tag="AGACAC" min_match="6" max_mismatch="0" match_edge="full">AGACAC</BASECALL> <BASECALL read_group_tag="ACGAAGTC" min_match="8" max_mismatch="0" match_edge="full">ACGAAGTC</BASECALL> <BASECALL read_group_tag="AACCGTGTC" min_match="9" max_mismatch="0" match_edge="full">AACCGTGTC</BASECALL> <BASECALL read_group_tag="TTAAGATTC" min_match="9" max_mismatch="0" match_edge="full">TTAAGATTC</BASECALL> <BASECALL read_group_tag="ACTCCTC" min_match="7" max_mismatch="0" match_edge="full">ACTCCTC</BASECALL> <BASECALL read_group_tag="CCTTCCGC" min_match="8" max_mismatch="0" match_edge="full">CCTTCCGC</BASECALL> <BASECALL read_group_tag="CGTGAC" min_match="6" max_mismatch="0" match_edge="full">CGTGAC</BASECALL> <BASECALL read_group_tag="ATACCAC" min_match="7" max_mismatch="0" match_edge="full">ATACCAC</BASECALL> <BASECALL read_group_tag="ACACGGAC" min_match="8" max_mismatch="0" match_edge="full">ACACGGAC</BASECALL> <BASECALL read_group_tag="ACCTTGGTC" min_match="9" max_mismatch="0" match_edge="full">ACCTTGGTC</BASECALL> <BASECALL read_group_tag="TGCGTTC" min_match="7" max_mismatch="0" match_edge="full">TGCGTTC</BASECALL> <BASECALL read_group_tag="TTGACAAC" min_match="8" max_mismatch="0" match_edge="full">TTGACAAC</BASECALL> <BASECALL read_group_tag="TCGAGGAAC" min_match="9" max_mismatch="0" match_edge="full">TCGAGGAAC</BASECALL> <BASECALL read_group_tag="AGACCTC" min_match="7" max_mismatch="0" match_edge="full">AGACCTC</BASECALL> <BASECALL read_group_tag="TGACGAC" min_match="7" max_mismatch="0" match_edge="full">TGACGAC</BASECALL> <BASECALL read_group_tag="TAGGAATC" min_match="8" max_mismatch="0" match_edge="full">TAGGAATC</BASECALL> <BASECALL read_group_tag="TCGTTGTC" min_match="8" max_mismatch="0" match_edge="full">TCGTTGTC</BASECALL> <BASECALL read_group_tag="ACTCAC" min_match="6" max_mismatch="0" match_edge="full">ACTCAC</BASECALL> </EXPECTED_BASECALL_TABLE> </READ_SPEC> <READ_SPEC> <READ_INDEX>2</READ_INDEX> <READ_LABEL>rRNA_primer</READ_LABEL> <READ_CLASS>Technical Read</READ_CLASS> <READ_TYPE>Primer</READ_TYPE> <EXPECTED_BASECALL_TABLE> <BASECALL read_group_tag="V1-V3" min_match="17" max_mismatch="0" match_edge="full">ATTACCGCGGCTGCTGG</BASECALL> <BASECALL read_group_tag="V3-V5" min_match="18" max_mismatch="0" match_edge="full">CCGTCAATTCMTTTRAGT</BASECALL> </EXPECTED_BASECALL_TABLE> </READ_SPEC> <READ_SPEC> <READ_INDEX>3</READ_INDEX> <READ_CLASS>Application Read</READ_CLASS> <READ_TYPE>Forward</READ_TYPE> <RELATIVE_ORDER follows_read_index="2"/> </READ_SPEC> </SPOT_DECODE_SPEC> </SPOT_DESCRIPTOR> </DESIGN> <PLATFORM> <LS454> <INSTRUMENT_MODEL>454 GS FLX Titanium</INSTRUMENT_MODEL> </LS454> </PLATFORM> <PROCESSING/> <EXPERIMENT_ATTRIBUTES> <EXPERIMENT_ATTRIBUTE> <TAG>library_strategy</TAG> <VALUE>targeted-locus</VALUE> </EXPERIMENT_ATTRIBUTE> <EXPERIMENT_ATTRIBUTE> <TAG>gene</TAG> <VALUE>16S rRNA V3-V5 region</VALUE> </EXPERIMENT_ATTRIBUTE> <EXPERIMENT_ATTRIBUTE> <TAG>gene</TAG> <VALUE>16S rRNA V1-V3 region</VALUE> </EXPERIMENT_ATTRIBUTE> </EXPERIMENT_ATTRIBUTES> </EXPERIMENT> </EXPERIMENT

SRX090194 112014 <STUDY_REF refname="phs000228_31" refcenter="NHGRI" accession="SRP002860"> <IDENTIFIERS> <PRIMARY_ID>SRP002860</PRIMARY_ID> <SUBMITTER_ID namespace="NHGRI">phs000228_31</SUBMITTER_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>LS454 sequencing of Human Microbiome Project 16S rRNA 454 Clinical Production Phase II 2855015837 fragment PCR library</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR refcenter="NCBI" accession="SRS011687"> <POOL> <DEFAULT_MEMBER> <IDENTIFIERS> <PRIMARY_ID>SRS011687</PRIMARY_ID> </IDENTIFIERS> </DEFAULT_MEMBER> <MEMBER member_name="2855230053_700103585_V1-V3" refname="700103585" refcenter="NCBI" accession="SRS043664"> <IDENTIFIERS> <PRIMARY_ID>SRS043664</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103585</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="ATACCAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230074_700105870_V1-V3" refname="700105870" refcenter="NCBI" accession="SRS055253"> <IDENTIFIERS> <PRIMARY_ID>SRS055253</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105870</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CGGTATC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230060_700105600_V3-V5" refname="700105600" refcenter="NCBI" accession="SRS054796"> <IDENTIFIERS> <PRIMARY_ID>SRS054796</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105600</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="AGTTGGC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230068_700105455_V3-V5" refname="700105455" refcenter="NCBI" accession="SRS051166"> <IDENTIFIERS> <PRIMARY_ID>SRS051166</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105455</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CTTCCTTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230080_700105872_V3-V5" refname="700105872" refcenter="NCBI" accession="SRS053092"> <IDENTIFIERS> <PRIMARY_ID>SRS053092</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105872</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TGACGAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230138_700105433_V1-V3" refname="700105433" refcenter="NCBI" accession="SRS044643"> <IDENTIFIERS> <PRIMARY_ID>SRS044643</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105433</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AAGGCAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230077_700105425_V3-V5" refname="700105425" refcenter="NCBI" accession="SRS055952"> <IDENTIFIERS> <PRIMARY_ID>SRS055952</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105425</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="ACTCCTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230061_700105230_V3-V5" refname="700105230" refcenter="NCBI" accession="SRS042523"> <IDENTIFIERS> <PRIMARY_ID>SRS042523</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105230</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="CCTTCCGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230072_700105447_V3-V5" refname="700105447" refcenter="NCBI" accession="SRS056523"> <IDENTIFIERS> <PRIMARY_ID>SRS056523</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105447</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TGCCGAAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230076_700106665_V3-V5" refname="700106665" refcenter="NCBI" accession="SRS056795"> <IDENTIFIERS> <PRIMARY_ID>SRS056795</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106665</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="CACGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230081_700106679_V1-V3" refname="700106679" refcenter="NCBI" accession="SRS054954"> <IDENTIFIERS> <PRIMARY_ID>SRS054954</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106679</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CGTGAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230051_700105715_V3-V5" refname="700105715" refcenter="NCBI" accession="SRS054477"> <IDENTIFIERS> <PRIMARY_ID>SRS054477</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105715</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TCTCGAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230050_700114068_V3-V5" refname="700114068" refcenter="NCBI" accession="SRS063070"> <IDENTIFIERS> <PRIMARY_ID>SRS063070</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700114068</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TGACCGTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230141_700106362_V3-V5" refname="700106362" refcenter="NCBI" accession="SRS050055"> <IDENTIFIERS> <PRIMARY_ID>SRS050055</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106362</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AGGCGGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230143_700103637_V3-V5" refname="700103637" refcenter="NCBI" accession="SRS044526"> <IDENTIFIERS> <PRIMARY_ID>SRS044526</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103637</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TTAAGATTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230140_700103448_V3-V5" refname="700103448" refcenter="NCBI" accession="SRS048692"> <IDENTIFIERS> <PRIMARY_ID>SRS048692</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103448</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AGGTTGTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230075_700106633_V1-V3" refname="700106633" refcenter="NCBI" accession="SRS045165"> <IDENTIFIERS> <PRIMARY_ID>SRS045165</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106633</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CGGTCTTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230073_700105604_V1-V3" refname="700105604" refcenter="NCBI" accession="SRS058326"> <IDENTIFIERS> <PRIMARY_ID>SRS058326</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105604</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="AACCTGGC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230057_700105769_V3-V5" refname="700105769" refcenter="NCBI" accession="SRS044569"> <IDENTIFIERS> <PRIMARY_ID>SRS044569</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105769</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TCACCTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230136_700106645_V1-V3" refname="700106645" refcenter="NCBI" accession="SRS055669"> <IDENTIFIERS> <PRIMARY_ID>SRS055669</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106645</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="ACTAATTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230058_700105858_V3-V5" refname="700105858" refcenter="NCBI" accession="SRS049858"> <IDENTIFIERS> <PRIMARY_ID>SRS049858</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105858</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CAAGAAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230055_700114023_V1-V3" refname="700114023" refcenter="NCBI" accession="SRS063248"> <IDENTIFIERS> <PRIMARY_ID>SRS063248</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700114023</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TGAAGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230054_700105741_V1-V3" refname="700105741" refcenter="NCBI" accession="SRS058170"> <IDENTIFIERS> <PRIMARY_ID>SRS058170</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105741</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AGACCTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230065_700106693_V1-V3" refname="700106693" refcenter="NCBI" accession="SRS049575"> <IDENTIFIERS> <PRIMARY_ID>SRS049575</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106693</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="ACTCAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230070_700105592_V1-V3" refname="700105592" refcenter="NCBI" accession="SRS051269"> <IDENTIFIERS> <PRIMARY_ID>SRS051269</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105592</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="AGCTTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230059_700105598_V1-V3" refname="700105598" refcenter="NCBI" accession="SRS051929"> <IDENTIFIERS> <PRIMARY_ID>SRS051929</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105598</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CGACTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230067_700105254_V3-V5" refname="700105254" refcenter="NCBI" accession="SRS045212"> <IDENTIFIERS> <PRIMARY_ID>SRS045212</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105254</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="ACCTTGGTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230064_700103452_V1-V3" refname="700103452" refcenter="NCBI" accession="SRS057461"> <IDENTIFIERS> <PRIMARY_ID>SRS057461</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103452</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="ACGAGAAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230134_700103243_V3-V5" refname="700103243" refcenter="NCBI" accession="SRS053264"> <IDENTIFIERS> <PRIMARY_ID>SRS053264</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103243</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TGCGTTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230052_700106906_V3-V5" refname="700106906" refcenter="NCBI" accession="SRS042558"> <IDENTIFIERS> <PRIMARY_ID>SRS042558</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700106906</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="CCTCTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230048_700105648_V1-V3" refname="700105648" refcenter="NCBI" accession="SRS051098"> <IDENTIFIERS> <PRIMARY_ID>SRS051098</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105648</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="ACACGGAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230135_700114141_V1-V3" refname="700114141" refcenter="NCBI" accession="SRS063236"> <IDENTIFIERS> <PRIMARY_ID>SRS063236</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700114141</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TTGACAAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230062_700105590_V3-V5" refname="700105590" refcenter="NCBI" accession="SRS043369"> <IDENTIFIERS> <PRIMARY_ID>SRS043369</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105590</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TATCAAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230082_700105284_V3-V5" refname="700105284" refcenter="NCBI" accession="SRS055631"> <IDENTIFIERS> <PRIMARY_ID>SRS055631</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105284</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AGACAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230049_700105992_V3-V5" refname="700105992" refcenter="NCBI" accession="SRS042788"> <IDENTIFIERS> <PRIMARY_ID>SRS042788</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105992</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TTCGAGC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230069_700105656_V3-V5" refname="700105656" refcenter="NCBI" accession="SRS046281"> <IDENTIFIERS> <PRIMARY_ID>SRS046281</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105656</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="CGTCGTC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230056_700103623_V1-V3" refname="700103623" refcenter="NCBI" accession="SRS051029"> <IDENTIFIERS> <PRIMARY_ID>SRS051029</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103623</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="TCGAGGAAC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230063_700103456_V3-V5" refname="700103456" refcenter="NCBI" accession="SRS047354"> <IDENTIFIERS> <PRIMARY_ID>SRS047354</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103456</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TCGTTGTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230142_700103462_V3-V5" refname="700103462" refcenter="NCBI" accession="SRS058246"> <IDENTIFIERS> <PRIMARY_ID>SRS058246</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103462</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TTGGAGGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230071_700105606_V1-V3" refname="700105606" refcenter="NCBI" accession="SRS056058"> <IDENTIFIERS> <PRIMARY_ID>SRS056058</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105606</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="ACGAAGTC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230066_700103710_V3-V5" refname="700103710" refcenter="NCBI" accession="SRS043411"> <IDENTIFIERS> <PRIMARY_ID>SRS043411</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103710</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TTCGTGGC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230139_700103587_V1-V3" refname="700103587" refcenter="NCBI" accession="SRS044606"> <IDENTIFIERS> <PRIMARY_ID>SRS044606</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103587</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="V1-V3">rRNA_primer</READ_LABEL> <READ_LABEL read_group_tag="AAGCCGC">barcode</READ_LABEL> </MEMBER> <MEMBER member_name="2855230137_700103702_V3-V5" refname="700103702" refcenter="NCBI" accession="SRS056473"> <IDENTIFIERS> <PRIMARY_ID>SRS056473</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700103702</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="AATGGTAC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> <MEMBER member_name="2855230083_700105642_V3-V5" refname="700105642" refcenter="NCBI" accession="SRS058322"> <IDENTIFIERS> <PRIMARY_ID>SRS058322</PRIMARY_ID> <SUBMITTER_ID namespace="NCBI">700105642</SUBMITTER_ID> </IDENTIFIERS> <READ_LABEL read_group_tag="TAGGAATC">barcode</READ_LABEL> <READ_LABEL read_group_tag="V3-V5">rRNA_primer</READ_LABEL> </MEMBER> </POOL> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>2855015837</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <SINGLE/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> <SPOT_DESCRIPTOR> <SPOT_DECODE_SPEC> <READ_SPEC> <READ_INDEX>0</READ_INDEX> <READ_CLASS>Technical Read</READ_CLASS> <READ_TYPE>Adapter</READ_TYPE> <BASE_COORD>1</BASE_COORD> </READ_SPEC> <READ_SPEC> <READ_INDEX>1</READ_INDEX> <READ_LABEL>barcode</READ_LABEL> <READ_CLASS>Technical Read</READ_CLASS> <READ_TYPE>BarCode</READ_TYPE> <EXPECTED_BASECALL_TABLE> <BASECALL read_group_tag="AACCTGGC" min_match="8" max_mismatch="0" match_edge="full">AACCTGGC</BASECALL> <BASECALL read_group_tag="CGTCGTC" min_match="7" max_mismatch="0" match_edge="full">CGTCGTC</BASECALL> <BASECALL read_group_tag="TGCCGAAC" min_match="8" max_mismatch="0" match_edge="full">TGCCGAAC</BASECALL> <BASECALL read_group_tag="CGGTCTTC" min_match="8" max_mismatch="0" match_edge="full">CGGTCTTC</BASECALL> <BASECALL read_group_tag="ACTAATTC" min_match="8" max_mismatch="0" match_edge="full">ACTAATTC</BASECALL> <BASECALL read_group_tag="AAGGCAC" min_match="7" max_mismatch="0" match_edge="full">AAGGCAC</BASECALL> <BASECALL read_group_tag="TGACCGTC" min_match="8" max_mismatch="0" match_edge="full">TGACCGTC</BASECALL> <BASECALL read_group_tag="TTGGAGGC" min_match="8" max_mismatch="0" match_edge="full">TTGGAGGC</BASECALL> <BASECALL read_group_tag="ACGAGAAC" min_match="8" max_mismatch="0" match_edge="full">ACGAGAAC</BASECALL> <BASECALL read_group_tag="TCACCTC" min_match="7" max_mismatch="0" match_edge="full">TCACCTC</BASECALL> <BASECALL read_group_tag="AGGCGGC" min_match="7" max_mismatch="0" match_edge="full">AGGCGGC</BASECALL> <BASECALL read_group_tag="AGGTTGTC" min_match="8" max_mismatch="0" match_edge="full">AGGTTGTC</BASECALL> <BASECALL read_group_tag="CGGTATC" min_match="7" max_mismatch="0" match_edge="full">CGGTATC</BASECALL> <BASECALL read_group_tag="TATCAAC" min_match="7" max_mismatch="0" match_edge="full">TATCAAC</BASECALL> <BASECALL read_group_tag="CTTCCTTC" min_match="8" max_mismatch="0" match_edge="full">CTTCCTTC</BASECALL> <BASECALL read_group_tag="CCTCTC" min_match="6" max_mismatch="0" match_edge="full">CCTCTC</BASECALL> <BASECALL read_group_tag="TTCGAGC" min_match="7" max_mismatch="0" match_edge="full">TTCGAGC</BASECALL> <BASECALL read_group_tag="AGTTGGC" min_match="7" max_mismatch="0" match_edge="full">AGTTGGC</BASECALL> <BASECALL read_group_tag="CGACTC" min_match="6" max_mismatch="0" match_edge="full">CGACTC</BASECALL> <BASECALL read_group_tag="AGCTTC" min_match="6" max_mismatch="0" match_edge="full">AGCTTC</BASECALL> <BASECALL read_group_tag="CACGC" min_match="5" max_mismatch="0" match_edge="full">CACGC</BASECALL> <BASECALL read_group_tag="AATGGTAC" min_match="8" max_mismatch="0" match_edge="full">AATGGTAC</BASECALL> <BASECALL read_group_tag="AAGCCGC" min_match="7" max_mismatch="0" match_edge="full">AAGCCGC</BASECALL> <BASECALL read_group_tag="TCTCGAC" min_match="7" max_mismatch="0" match_edge="full">TCTCGAC</BASECALL> <BASECALL read_group_tag="TGAAGC" min_match="6" max_mismatch="0" match_edge="full">TGAAGC</BASECALL> <BASECALL read_group_tag="CAAGAAC" min_match="7" max_mismatch="0" match_edge="full">CAAGAAC</BASECALL> <BASECALL read_group_tag="TTCGTGGC" min_match="8" max_mismatch="0" match_edge="full">TTCGTGGC</BASECALL> <BASECALL read_group_tag="AGACAC" min_match="6" max_mismatch="0" match_edge="full">AGACAC</BASECALL> <BASECALL read_group_tag="ACGAAGTC" min_match="8" max_mismatch="0" match_edge="full">ACGAAGTC</BASECALL> <BASECALL read_group_tag="AACCGTGTC" min_match="9" max_mismatch="0" match_edge="full">AACCGTGTC</BASECALL> <BASECALL read_group_tag="TTAAGATTC" min_match="9" max_mismatch="0" match_edge="full">TTAAGATTC</BASECALL> <BASECALL read_group_tag="ACTCCTC" min_match="7" max_mismatch="0" match_edge="full">ACTCCTC</BASECALL> <BASECALL read_group_tag="CCTTCCGC" min_match="8" max_mismatch="0" match_edge="full">CCTTCCGC</BASECALL> <BASECALL read_group_tag="CGTGAC" min_match="6" max_mismatch="0" match_edge="full">CGTGAC</BASECALL> <BASECALL read_group_tag="ATACCAC" min_match="7" max_mismatch="0" match_edge="full">ATACCAC</BASECALL> <BASECALL read_group_tag="ACACGGAC" min_match="8" max_mismatch="0" match_edge="full">ACACGGAC</BASECALL> <BASECALL read_group_tag="ACCTTGGTC" min_match="9" max_mismatch="0" match_edge="full">ACCTTGGTC</BASECALL> <BASECALL read_group_tag="TGCGTTC" min_match="7" max_mismatch="0" match_edge="full">TGCGTTC</BASECALL> <BASECALL read_group_tag="TTGACAAC" min_match="8" max_mismatch="0" match_edge="full">TTGACAAC</BASECALL> <BASECALL read_group_tag="TCGAGGAAC" min_match="9" max_mismatch="0" match_edge="full">TCGAGGAAC</BASECALL> <BASECALL read_group_tag="AGACCTC" min_match="7" max_mismatch="0" match_edge="full">AGACCTC</BASECALL> <BASECALL read_group_tag="TGACGAC" min_match="7" max_mismatch="0" match_edge="full">TGACGAC</BASECALL> <BASECALL read_group_tag="TAGGAATC" min_match="8" max_mismatch="0" match_edge="full">TAGGAATC</BASECALL> <BASECALL read_group_tag="TCGTTGTC" min_match="8" max_mismatch="0" match_edge="full">TCGTTGTC</BASECALL> <BASECALL read_group_tag="ACTCAC" min_match="6" max_mismatch="0" match_edge="full">ACTCAC</BASECALL> </EXPECTED_BASECALL_TABLE> </READ_SPEC> <READ_SPEC> <READ_INDEX>2</READ_INDEX> <READ_LABEL>rRNA_primer</READ_LABEL> <READ_CLASS>Technical Read</READ_CLASS> <READ_TYPE>Primer</READ_TYPE> <EXPECTED_BASECALL_TABLE> <BASECALL read_group_tag="V1-V3" min_match="17" max_mismatch="0" match_edge="full">ATTACCGCGGCTGCTGG</BASECALL> <BASECALL read_group_tag="V3-V5" min_match="18" max_mismatch="0" match_edge="full">CCGTCAATTCMTTTRAGT</BASECALL> </EXPECTED_BASECALL_TABLE> </READ_SPEC> <READ_SPEC> <READ_INDEX>3</READ_INDEX> <READ_CLASS>Application Read</READ_CLASS> <READ_TYPE>Forward</READ_TYPE> <RELATIVE_ORDER follows_read_index="2"/> </READ_SPEC> </SPOT_DECODE_SPEC> </SPOT_DESCRIPTOR> </DESIGN> <PLATFORM> <LS454> <INSTRUMENT_MODEL>454 GS FLX Titanium</INSTRUMENT_MODEL> </LS454> </PLATFORM> <PROCESSING/> <EXPERIMENT_ATTRIBUTES> <EXPERIMENT_ATTRIBUTE> <TAG>library_strategy</TAG> <VALUE>targeted-locus</VALUE> </EXPERIMENT_ATTRIBUTE> <EXPERIMENT_ATTRIBUTE> <TAG>gene</TAG> <VALUE>16S rRNA V3-V5 region</VALUE> </EXPERIMENT_ATTRIBUTE> <EXPERIMENT_ATTRIBUTE> <TAG>gene</TAG> <VALUE>16S rRNA V1-V3 region</VALUE> </EXPERIMENT_ATTRIBUTE> </EXPERIMENT_ATTRIBUTES> </EXPERIMENT> </EXPERIMENT_SET>