SRS255950 SAMN00704054 496920 saltern metagenome The sample was collected from Bras del Port salterns in Alicante, Spain . The concentrator pond (SS19) was sampled on July 21, 2008. The salinity was measured using a hand refractometer (salinity 19%). The sample was sequentially filtered through a 5 micron and 0.22 micron-pore size polycarbonate filters using a peristaltic pump. Filters were stored at -80 deg C until DNA extraction. DNA was extracted as described before (Martin-Cuadrado et al, 2007), briefly, filters were thawed on ice and then treated with 1 mg/ml lysozyme and 0.2 mg/ml proteinase K (final concentrations). Nucleic acids were extracted with phenol/chloroform/isoamyl alcohol and chloroform/isoamyl alcohol and DNA integrity was checked by agarose gel electrophoresis. 5 micrograms of DNA sent separately for sequencing (Roche 454 GS-FLX system, Titanium chemistry, by GATC, Konstanz, Germany) (one full plate 454). SRS255951 SAMN00704055 496920 saltern metagenome The sample was collected from Bras del Port salterns in Alicante, Spain . The concentrator pond (SS19) was sampled on July 21, 2008. The salinity was measured using a hand refractometer (salinity 19%). The sample was sequentially filtered through a 5 micron and 0.22 micron-pore size polycarbonate filters using a peristaltic pump. Filters were stored at -80 deg C until DNA extraction. DNA was extracted as described before (Martin-Cuadrado et al, 2007), briefly, filters were thawed on ice and then treated with 1 mg/ml lysozyme and 0.2 mg/ml proteinase K (final concentrations). Nucleic acids were extracted with phenol/chloroform/isoamyl alcohol and chloroform/isoamyl alcohol and DNA integrity was checked by agarose gel electrophoresis. 5 micrograms of DNA sent separately for sequencing (Roche 454 GS-FLX system, Titanium chemistry, by GATC, Konstanz, Germany) (one full plate 454).