SRX090767 b_napus_14_20_exp SRP007759 brassica_napus_msu_454 Total RNA from frozen tissue of B. napus was extracted using TRIzol Reagent and mRNA was purified using oligo dT purification kit. cDNA synthesis was with Creator SMART cDNA Library Construction Kit (Clontech), using LD PCR. First strand cDNA was synthesized from 2.0 *g mRNA using SUPERSCRIPT II reverse transcriptase (Invitrogen), and CDS III /3’ primer (5’tagaggccgaggcggccgacatgttttgtttttttttcttttttttttvn3’). Sequencing with 454 Titanium. SRS256234 b_napus_14_20 FMEGV6E EST TRANSCRIPTOMIC cDNA 800 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 454 Basecaller Base Caller 454 Basecaller Quality Scorer SRX090768 b_napus_21_25_exp SRP007759 brassica_napus_msu_454 Total RNA from frozen tissue of B. napus was extracted using TRIzol Reagent and mRNA was purified using oligo dT purification kit. cDNA synthesis was with Creator SMART cDNA Library Construction Kit (Clontech), using LD PCR. First strand cDNA was synthesized from 2.0 *g mRNA using SUPERSCRIPT II reverse transcriptase (Invitrogen), and CDS III /3’ primer (5’tagaggccgaggcggccgacatgttttgtttttttttcttttttttttvn3’). Sequencing with 454 Titanium. SRS256235 b_napus_21_25 FMRAAFF EST TRANSCRIPTOMIC cDNA 800 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 454 Basecaller Base Caller 454 Basecaller Quality Scorer SRX090769 b_napus_26_30_exp SRP007759 brassica_napus_msu_454 Total RNA from frozen tissue of B. napus was extracted using TRIzol Reagent and mRNA was purified using oligo dT purification kit. cDNA synthesis was with Creator SMART cDNA Library Construction Kit (Clontech), using LD PCR. First strand cDNA was synthesized from 2.0 *g mRNA using SUPERSCRIPT II reverse transcriptase (Invitrogen), and CDS III /3’ primer (5’tagaggccgaggcggccgacatgttttgtttttttttcttttttttttvn3’). Sequencing with 454 Titanium. SRS256236 b_napus_26_30 FO4H2WP EST TRANSCRIPTOMIC cDNA 800 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 454 Basecaller Base Caller 454 Basecaller Quality Scorer SRX090771 b_napus_31_35_exp SRP007759 brassica_napus_msu_454 Total RNA from frozen tissue of B. napus was extracted using TRIzol Reagent and mRNA was purified using oligo dT purification kit. cDNA synthesis was with Creator SMART cDNA Library Construction Kit (Clontech), using LD PCR. First strand cDNA was synthesized from 2.0 *g mRNA using SUPERSCRIPT II reverse transcriptase (Invitrogen), and CDS III /3’ primer (5’tagaggccgaggcggccgacatgttttgtttttttttcttttttttttvn3’). Sequencing with 454 Titanium. SRS256237 b_napus_31_35 FO785IJ EST TRANSCRIPTOMIC cDNA 800 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium 454 Basecaller Base Caller 454 Basecaller Quality Scorer