SRS256172 SAMN00704258 7160 Aedes albopictus We collected over 400 Ae. albopictus larvae and pupae from approximately 20 tires located at a used tire yard in Manassas, VA, in 2008. This strain was reared in the laboratory on a non-diapause inducing (NDI) long-day photoperiod (16 h light, 8 h dark) for five generations as described in Armbruster and Hutchinson (2002) and Armbruster and Conn (2006). To produce tissue for transcriptome sequencing, approximately 200 female pupae were placed into each of two cages, one of which was maintained under an NDI photoperiod (16 h light, 8 h dark) and the other of which was maintained under a diapause-inducing (DI) unambiguous short-day photoperiod (8 h light, 16 h dark). Both cages were maintained at 21oC and ca. 80% relative humidity and females were bloodfed 7-18 days after eclosion on a human host. Five days after bloodfeeding, females were anaesthetized with CO2 and frozen at -80oC. Mature (stage V) oocytes were identified based on a visible exochorion pattern and dissected dissected into RNAlater TM (Sigma Aldrich, St. Louis, MO). 60 – 80 frozen mosquitoes per photoperiod treatment were used. AlbopictusExpression http://www.albopictusexpression.org/ SRS256173 SAMN00704259 7160 Aedes albopictus We collected over 400 Ae. albopictus larvae and pupae from approximately 20 tires located at a used tire yard in Manassas, VA, in 2008. This strain was reared in the laboratory on a non-diapause inducing (NDI) long-day photoperiod (16 h light, 8 h dark) for five generations as described in Armbruster and Hutchinson (2002) and Armbruster and Conn (2006). To produce tissue for transcriptome sequencing, approximately 200 female pupae were placed into each of two cages, one of which was maintained under an NDI photoperiod (16 h light, 8 h dark) and the other of which was maintained under a diapause-inducing (DI) unambiguous short-day photoperiod (8 h light, 16 h dark). Both cages were maintained at 21oC and ca. 80% relative humidity and females were bloodfed 7-18 days after eclosion on a human host. Five days after bloodfeeding, females were anaesthetized with CO2 and frozen at -80oC. Mature (stage V) oocytes were identified based on a visible exochorion pattern and dissected dissected into RNAlater TM (Sigma Aldrich, St. Louis, MO). 60 – 80 frozen mosquitoes per photoperiod treatment were used. AlbopictusExpression http://www.albopictusexpression.org/