Role of the Microbiota in Asthma, Run #2

SRX120174 Finlay 454 Run 2 Role of the Microbiota in Asthma, Run #2 SRP010946 Finlay Asthma 001 DNA was extracted from feces using a Qiagen Stool Kit. It was then PCR amplified using 16SrRNA primers specific for V1-V2 regions of the gene. Amplicons were gel purified and resuspended to a common concentration, then submitted for 454 pyrosequencing. SRS293512 Untreated-1 16S rRNA from Feces OTHER METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120331 Finlay Asthma 002 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293513 Untreated-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120332 Finlay Asthma 003 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293514 Untreated-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120333 Finlay Asthma 004 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293515 Untreated-4 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120334 Finlay Asthma 005 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293520 Strep-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120335 Finlay Asthma 006 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293521 Strep-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120336 Finlay Asthma 007 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293522 Strep-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120337 Finlay Asthma 008 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293523 Strep-4 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120338 Finlay Asthma 009 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293516 Vanco-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120339 Finlay Asthma 010 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293517 Vanco-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120340 Finlay Asthma 011 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293518 Vanco-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120341 Finlay Asthma 012 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293519 Vanco-4 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120342 Finlay Asthma 013 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293524 ST Untreated-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120343 Finlay Asthma 014 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293525 ST Untreated-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120344 Finlay Asthma 015 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293526 ST Untreated-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120345 Finlay Asthma 016 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293527 ST Untreated-4 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120346 Finlay Asthma 017 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293532 ST Strep-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120347 Finlay Asthma 018 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293533 ST Strep-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120348 Finlay Asthma 019 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293534 ST Strep-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120349 Finlay Asthma 020 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293535 ST Strep-4 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120350 Finlay Asthma 021 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293528 ST Vanco-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120351 Finlay Asthma 022 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293529 ST Vanco-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120352 Finlay Asthma 023 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293530 ST Vanco-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120353 Finlay Asthma 024 Short-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293531 ST Vanco-4 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120354 G75JS3M02 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293856 LT OVA Control-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120355 G75JS3M03 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293857 LT OVA IL Control-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120356 G75JS3M04 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293858 LT OVA Control-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120357 G75JS3M05 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293859 LT OVA IL Control-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120358 G75JS3M06 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293860 LT OVA Control-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120359 G75JS3M07 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293861 LT OVA IL Control-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120360 G75JS3M08 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293862 LT OVA Control-4 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120361 G75JS3M09 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293863 LT OVA IL Control-4 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120362 G75JS3M10 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293864 LT OVA Control-5 5 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120363 G75JS3M11 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293865 LT OVA IL Control-6 5 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120364 G75JS3M12 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293866 LT OVA IL Strep-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120365 G75JS3M13 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293867 LT OVA Strep-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120366 G75JS3M14 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293868 LT OVA Strep-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120367 G75JS3M15 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293869 LT OVA IL Strep-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120368 G75JS3M16 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293870 LT OVA Strep-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120369 G75JS3M17 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293871 LT OVA IL Strep-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120370 G75JS3M18 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293872 LT OVA Strep-4 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120371 G75JS3M19 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293873 LT OVA Strep-5 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120372 G75JS3M20 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293874 LT OVA IL Strep-5 5 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120373 G75JS3M21 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293875 LT OVA Vanco-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120374 G75JS3M22 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293876 LT OVA IL Vanco-1 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120375 G75JS3M23 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293877 LT OVA Vanco-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120376 G75JS3M24 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293878 LT OVA IL Vanco-2 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120377 G75JS3M25 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Feces SRS293879 LT OVA Vanco-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120378 G75JS3M26 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293880 LT OVA IL Vanco-3 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120379 G75JS3M27 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293881 LT OVA IL Vanco-4 4 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120380 G75JS3M28 Long-term Antibitoics SRP010946 Finlay Asthma 001 Post-Asthma Ileum SRS293882 LT OVA IL Vanco-5 5 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120381 G75JS3M29 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293883 LT Control-1B 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120382 G75JS3M30 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Ileum SRS293884 LT IL Control-1B 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120383 G75JS3M31 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293885 LT Control-2B 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120384 G75JS3M32 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Ileum SRS293886 LT IL Control-2B 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120385 G75JS3M33 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293887 LT Control-3B 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120386 G75JS3M34 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Ileum SRS293888 LT IL Control-3B 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120387 G75JS3M35 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293889 LT Strep-1B 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120388 G75JS3M36 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293890 LT Strep-2B 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120389 G75JS3M37 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Ileum SRS293891 LT IL Strep-2B 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120390 G75JS3M38 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293892 LT Strep-3B 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120391 G75JS3M39 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Ileum SRS293893 LT IL Strep-3B 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120392 G75JS3M40 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293894 LT Vanco-1B 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120393 G75JS3M41 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Ileum SRS293895 LT IL Vanco-1B 1 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120394 G75JS3M42 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293896 LT Vanco-2B 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120395 G75JS3M43 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Ileum SRS293897 LT IL Vanco-2B 2 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120396 G75JS3M44 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Feces SRS293898 LT Vanco-3B 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX120397 G75JS3M45 Long-term Antibitoics SRP010946 Finlay Asthma 001 Pre-Asthma Ileum SRS293899 LT IL Vanco-3B 3 OTHER METAGENOMIC PCR 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium