<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT alias="11B-884-1-leaf" center_name="Iowa State University" accession="SRX130104">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX130104</PRIMARY_ID>
      <SUBMITTER_ID namespace="Iowa State University">11B-884-1-leaf</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>Zea mays "Miami White" 14-day seedling leaf RNA-Seq (Schnable Lab Ac5684; Row #: 11B-884-1)</TITLE>
    <STUDY_REF accession="SRP011566">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP011566</PRIMARY_ID>
        <SUBMITTER_ID namespace="Iowa State University">NSF-CNV-Miami_White</SUBMITTER_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>All RNA extractions were conducted using the Qiagen RNeasy kit cat# 74904 (50), according to Qiagen's protocol. Total RNA was eluted twice with 30 ul RNase-free water. The indexed RNA-Seq libraries were prepared using the Illumina protocol outlined in "TruSeq RNA Sample Preparation Guide" (Part# 15008136 Rev. A November 2010). The indexed libraries from one inbred/line were combined and seeded onto one lane of the flowcell. The libraries were sequenced using 110 cycles of chemistry and imaging, resulting in paired-end (PE) sequencing reads with length of 2 x 101 bp.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS300835">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS300835</PRIMARY_ID>
          <SUBMITTER_ID namespace="Iowa State University">Miami White</SUBMITTER_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>11B-884-1-leaf</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
      <SPOT_DESCRIPTOR>
        <SPOT_DECODE_SPEC>
          <SPOT_LENGTH>202</SPOT_LENGTH>
          <READ_SPEC>
            <READ_INDEX>0</READ_INDEX>
            <READ_CLASS>Application Read</READ_CLASS>
            <READ_TYPE>Forward</READ_TYPE>
            <BASE_COORD>1</BASE_COORD>
          </READ_SPEC>
          <READ_SPEC>
            <READ_INDEX>1</READ_INDEX>
            <READ_CLASS>Application Read</READ_CLASS>
            <READ_TYPE>Reverse</READ_TYPE>
            <BASE_COORD>102</BASE_COORD>
          </READ_SPEC>
        </SPOT_DECODE_SPEC>
      </SPOT_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <PROCESSING/>
  </EXPERIMENT>
</EXPERIMENT_SET>
