SRX130252 GSM897555_2 SRP011584 GSE36616 SRS301487 GSM897555: Proliferating Histone 3 ChIP ChIP-Seq GENOMIC ChIP 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM897555 gds 300897555 GEO Accession GSM897555 SRX130253 GSM897556_2 SRP011584 GSE36616 SRS301488 GSM897556: Proliferating H3K4me3 ChIP ChIP-Seq GENOMIC ChIP 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM897556 gds 300897556 GEO Accession GSM897556 SRX130254 GSM897557_1 SRP011584 GSE36616 SRS301489 GSM897557: Proliferating H3K27me3 ChIP ChIP-Seq GENOMIC ChIP 50 0 Application Read Forward 1 Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM897557 gds 300897557 GEO Accession GSM897557 SRX130255 GSM897558_2 SRP011584 GSE36616 SRS301490 GSM897558: Proliferating LaminB1 ChIP ChIP-Seq GENOMIC ChIP 36 0 Application Read Forward 1 Illumina Genome Analyzer IIx GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM897558 gds 300897558 GEO Accession GSM897558 SRX130256 GSM897559_2 SRP011584 GSE36616 SRS301491 GSM897559: Senescent Histone 3 ChIP ChIP-Seq GENOMIC ChIP 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM897559 gds 300897559 GEO Accession GSM897559 SRX130257 GSM897560_2 SRP011584 GSE36616 SRS301492 GSM897560: Senescent H3K4me3 ChIP ChIP-Seq GENOMIC ChIP 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM897560 gds 300897560 GEO Accession GSM897560 SRX130258 GSM897561_1 SRP011584 GSE36616 SRS301493 GSM897561: Senescent H3K27me3 ChIP ChIP-Seq GENOMIC ChIP 50 0 Application Read Forward 1 Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM897561 gds 300897561 GEO Accession GSM897561 SRX275792 GSM1135038_1 SRP011584 GSE36616 SRS419344 GSM1135038 ChIP-Seq GENOMIC ChIP Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes. Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C. ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample. 30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures. Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively). Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1135038 gds 301135038 GEO Accession GSM1135038 SRX275793 GSM1135039_1 SRP011584 GSE36616 SRS419345 GSM1135039 ChIP-Seq GENOMIC ChIP Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes. Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C. ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample. 30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures. Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively). Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1135039 gds 301135039 GEO Accession GSM1135039 SRX275794 GSM1135040_1 SRP011584 GSE36616 SRS419346 GSM1135040 ChIP-Seq GENOMIC ChIP Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes. Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C. ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample. 30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures. Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively). Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1135040 gds 301135040 GEO Accession GSM1135040 SRX275795 GSM1135041_1 SRP011584 GSE36616 SRS419347 GSM1135041 ChIP-Seq GENOMIC ChIP Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes. Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C. ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample. 30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures. Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively). Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1135041 gds 301135041 GEO Accession GSM1135041 SRX275796 GSM1135042_1 SRP011584 GSE36616 SRS419348 GSM1135042 ChIP-Seq GENOMIC ChIP Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes. Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C. ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample. 30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures. Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively). Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1135042 gds 301135042 GEO Accession GSM1135042 SRX275797 GSM1135043_1 SRP011584 GSE36616 SRS419349 GSM1135043 ChIP-Seq GENOMIC ChIP Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes. Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C. ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample. 30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures. Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively). Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1135043 gds 301135043 GEO Accession GSM1135043 SRX275798 GSM1135044_1 SRP011584 GSE36616 SRS419350 GSM1135044 ChIP-Seq GENOMIC ChIP Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes. Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C. ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample. 30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures. Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively). Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1135044 gds 301135044 GEO Accession GSM1135044 SRX275799 GSM1135045_1 SRP011584 GSE36616 SRS419351 GSM1135045 ChIP-Seq GENOMIC ChIP Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes. Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C. ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample. 30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures. Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively). Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1135045 gds 301135045 GEO Accession GSM1135045 SRX275800 GSM1135046_1 SRP011584 GSE36616 SRS419352 GSM1135046 ChIP-Seq GENOMIC ChIP Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes. Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C. ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample. 30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures. Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively). Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1135046 gds 301135046 GEO Accession GSM1135046 SRX275801 GSM1135047_1 SRP011584 GSE36616 SRS419353 GSM1135047 ChIP-Seq GENOMIC ChIP Cells in 10cm2 dishes were fixed in 1% formaldehyde for 10 minutes and fixation was quenched with addition of glycine to 125mM for an additional 5 minutes. Cells were harvested by scraping from plates, and washed twice in 1xPBS before storage at -80C. ChIP was performed as described in the Young lab protocol, except that extracts were sonicated twice for 9 minutes each round (30 seconds sonication with intermediate incubation of 30 seconds per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500µg of extract 2µg of antibody per sample. 30µl of Protein G Dynabeads (Invitrogen 100.02D) were used per ChIP. For sequencing, 10ng ChIP DNA was used to make sequencing libraries using standard Illumina library single-end construction procedures. Sequencing was performed on either Illumina GAIIx or Hi-Seq platforms (36bp, single-end and 50 or 100bp, single or paired-end reads, respectively). Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1135047 gds 301135047 GEO Accession GSM1135047