<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT alias="SRA058763" accession="SRX255777" center_name="P&amp;G; QIBEBT,CAS,China">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX255777</PRIMARY_ID>
      <SUBMITTER_ID namespace="P&amp;G; QIBEBT,CAS,China">SRA058763</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>Predictive modeling of gingivitis severity and susceptibility via oral microbiota</TITLE>
    <STUDY_REF accession="SRP022235">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP022235</PRIMARY_ID>
        <SUBMITTER_ID namespace="BioProject">PRJNA194521</SUBMITTER_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>For 18 of the plaques metagenomic DNA were separately extracted and sequenced. The samples were at both Baseline and EG and from nine of the subjects. The paired-end sequencing libraries were prepared under the NEXTflexTM technology (BIO Scientific Corp., USA). Metagenomic DNA was first fragmented with liquid nitrogen. Sequencing adaptors that include the index sequences were then ligated on the size-selected fragments. Ten cycles of PCR were introduced to enrich the properly ligated fragments. The enriched products were then sequenced on HiSeq (Illumina, USA) with 2 x120bp read length. These reads were subjected to quality filtering, and then human reads identified and separately archived.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS577934">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS577934</PRIMARY_ID>
          <SUBMITTER_ID namespace="Qibebt,CAS,China">PG.fq</SUBMITTER_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>P&amp;G gingivitis microbiota</LIBRARY_NAME>
        <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>unspecified</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
      <SPOT_DESCRIPTOR>
        <SPOT_DECODE_SPEC>
          <SPOT_LENGTH>120</SPOT_LENGTH>
          <READ_SPEC>
            <READ_INDEX>0</READ_INDEX>
            <READ_CLASS>Application Read</READ_CLASS>
            <READ_TYPE>Forward</READ_TYPE>
            <BASE_COORD>1</BASE_COORD>
          </READ_SPEC>
        </SPOT_DECODE_SPEC>
      </SPOT_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <PROCESSING/>
  </EXPERIMENT>
</EXPERIMENT_SET>
