High-throughput RNA sequencing of a FFPE autopsy lung tissue samples from the 2009 influenza pandemic

SRX203189 2009influenzaFFPE High-throughput RNA sequencing of a FFPE autopsy lung tissue samples from the 2009 influenza pandemic SRP017105 The construction of total RNA libraries from FFPE samples was performed according to Illumina mRNA-seq sample preparation kit (Illumina, San Diego, CA) specifications but skipping the poly-A selection and RNA fragmentation steps. Then, 2 rounds of Duplex-Specific Nuclease (DSN) (Evrogen, Moscow, Russia) treatments on the library were performed according to the Illumina DSN normalization procedure. SRS374422 RNA-Seq METATRANSCRIPTOMIC RANDOM 76 0 Application Read Forward 1 Illumina Genome Analyzer IIx SRX203200 1918influenzaFFPE High-throughput RNA sequencing of a FFPE autopsy lung tissue sample from the 1918 influenza pandemic SRP017105 PRJNA178740 The construction of total RNA libraries from FFPE samples was performed according to Illumina mRNA-seq sample preparation kit (Illumina, San Diego, CA) specifications but skipping the poly-A selection and RNA fragmentation steps. Then, 2 rounds of Duplex-Specific Nuclease (DSN) (Evrogen, Moscow, Russia) treatments were performed on the library according to the Illumina DSN normalization procedure. SRS374432 SAMN01801701 RNA-Seq METATRANSCRIPTOMIC RANDOM 36 0 Application Read Forward 1 Illumina Genome Analyzer IIx SRX8181170 RNA-seq of A549 cell line RNA-seq of A549 cell line SRP017105 PRJNA178740 The construction of mRNA library from mock-infected cultured human lung alveolar epithelial cells (A549 cell line) was performed according to Illumina mRNA-seq sample preparation kit (Illumina, San Diego, CA) specifications. SRS6540240 SAMN14681245 FC64YW4AAXX RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina Genome Analyzer IIx