<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT accession="SRX204381" center_name="MBRI" alias="MethylDOnorCOlitis">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX204381</PRIMARY_ID>
      <SUBMITTER_ID namespace="MBRI">MethylDOnorCOlitis</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>Prenatal methyl-donor supplementation induces an acute colitis prone microbiome in young adult mice</TITLE>
    <STUDY_REF accession="SRP017164">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP017164</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Utilizing barcoded amplicon sequencing PCR was performed with the common primers 515F-806R for bacterial diversity.  Sequences were derived from ion torrent PGM sequencing method  #SampleID BarcodeSequence LinkerPrimerSequence BarcodeName ProjectName Description Reads  C3aRRM TCGAGCTC GTGCCAGCMGCCGCGGTAA bac515FION1 072712keller C3aRRM 10k  C3aRRF TCGCAAGT GTGCCAGCMGCCGCGGTAA bac515FION2 072712keller C3aRRF 10k  C8a0M TCGCTAAT GTGCCAGCMGCCGCGGTAA bac515FION3 072712keller C8a0M 10k  C8a0F TCGCTGAC GTGCCAGCMGCCGCGGTAA bac515FION4 072712keller C8a0F 10k  C7aRM TCGGAACA GTGCCAGCMGCCGCGGTAA bac515FION5 072712keller C7aRM 10k  C7aRF TCGGAAGA GTGCCAGCMGCCGCGGTAA bac515FION6 072712keller C7aRF 10k  C7aLM TCGGACCT GTGCCAGCMGCCGCGGTAA bac515FION7 072712keller C7aLM 10k  C7aLF TCGGGCCT GTGCCAGCMGCCGCGGTAA bac515FION8 072712keller C7aLF 10k  C7a0M TCGTATGG GTGCCAGCMGCCGCGGTAA bac515FION9 072712keller C7a0M 10k  C7a0F TCGTCCTA GTGCCAGCMGCCGCGGTAA bac515FION10 072712keller C7a0F 10k  C2aRM TCGTCGAC GTGCCAGCMGCCGCGGTAA bac515FION11 072712keller C2aRM 10k  C2aRF TCGTTACT GTGCCAGCMGCCGCGGTAA bac515FION12 072712keller C2aRF 10k  C2aLM TCTAACTA GTGCCAGCMGCCGCGGTAA bac515FION13 072712keller C2aLM 10k  C2aLF TCTAAGAG GTGCCAGCMGCCGCGGTAA bac515FION14 072712keller C2aLF 10k  C1bRM TCTAATAC GTGCCAGCMGCCGCGGTAA bac515FION15 072712keller C1bRM 10k  C1bRF TCTACAGA GTGCCAGCMGCCGCGGTAA bac515FION16 072712keller C1bRF 10k  C1bLM TCTACCAC GTGCCAGCMGCCGCGGTAA bac515FION17 072712keller C1bLM 10k  C1bLF TCTACGCA GTGCCAGCMGCCGCGGTAA bac515FION18 072712keller C1bLF 10k  C1b0M TCTAGATA GTGCCAGCMGCCGCGGTAA bac515FION19 072712keller C1b0M 10k  C1b0F TCTAGCTG GTGCCAGCMGCCGCGGTAA bac515FION20 072712keller C1b0F 10k</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS375287">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS375287</PRIMARY_ID>
          <SUBMITTER_ID namespace="MBRI">MethylDOnorCOlitis</SUBMITTER_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME/>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
      <SPOT_DESCRIPTOR>
        <SPOT_DECODE_SPEC>
          <SPOT_LENGTH>0</SPOT_LENGTH>
          <READ_SPEC>
            <READ_INDEX>0</READ_INDEX>
            <READ_CLASS>Technical Read</READ_CLASS>
            <READ_TYPE>Adapter</READ_TYPE>
            <BASE_COORD>1</BASE_COORD>
          </READ_SPEC>
          <READ_SPEC>
            <READ_INDEX>1</READ_INDEX>
            <READ_CLASS>Application Read</READ_CLASS>
            <READ_TYPE>Forward</READ_TYPE>
            <BASE_COORD>5</BASE_COORD>
          </READ_SPEC>
        </SPOT_DECODE_SPEC>
      </SPOT_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ION_TORRENT>
        <INSTRUMENT_MODEL>Ion Torrent PGM</INSTRUMENT_MODEL>
      </ION_TORRENT>
    </PLATFORM>
    <PROCESSING/>
  </EXPERIMENT>
</EXPERIMENT_SET>
