SRX205680 Control 1 SRP016086 Poly(A)+ selection, mRNA library construction, cluster generation and sequencing was conducted by Macrogen Inc., Seoul, Korea. RNA samples were prepared for sequencing using a TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s instructions. mRNA was purified from total RNA by way of poly (A)+ selection before samples were fragmented and reverse transcribed to cDNA using random hexamer priming. Following fragment end-repair, individual frog cDNA libraries were tagged by ligation of unique indexing adapters to cDNA ends in preparation for hybridisation onto a flow cell. PCR was then used to enrich for adapter-containing cDNAs followed by quality control analysis of each sample library and quantification of the DNA library templates. Prior to sequencing, DNA templates were bridge-amplified to produce clonal clusters on the surface of the flow cell. Sequencing was then carried out using an Illumina HiSeq 2000. SRS376454 Control 1 RNA-Seq TRANSCRIPTOMIC unspecified 210 0 Application Read Forward 1 1 Application Read Reverse 106 Illumina HiSeq 2000 Illumina Pipeline (CASAVA) v1.8.2 SRX205682 Control 2 SRP016086 Poly(A)+ selection, mRNA library construction, cluster generation and sequencing was conducted by Macrogen Inc., Seoul, Korea. RNA samples were prepared for sequencing using a TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s instructions. mRNA was purified from total RNA by way of poly (A)+ selection before samples were fragmented and reverse transcribed to cDNA using random hexamer priming. Following fragment end-repair, individual frog cDNA libraries were tagged by ligation of unique indexing adapters to cDNA ends in preparation for hybridisation onto a flow cell. PCR was then used to enrich for adapter-containing cDNAs followed by quality control analysis of each sample library and quantification of the DNA library templates. Prior to sequencing, DNA templates were bridge-amplified to produce clonal clusters on the surface of the flow cell. Sequencing was then carried out using an Illumina HiSeq 2000. SRS376455 Control 2 RNA-Seq TRANSCRIPTOMIC unspecified 210 0 Application Read Forward 1 1 Application Read Reverse 106 Illumina HiSeq 2000 Illumina Pipeline (CASAVA) v1.8.2 SRX205683 Control 6 SRP016086 Poly(A)+ selection, mRNA library construction, cluster generation and sequencing was conducted by Macrogen Inc., Seoul, Korea. RNA samples were prepared for sequencing using a TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s instructions. mRNA was purified from total RNA by way of poly (A)+ selection before samples were fragmented and reverse transcribed to cDNA using random hexamer priming. Following fragment end-repair, individual frog cDNA libraries were tagged by ligation of unique indexing adapters to cDNA ends in preparation for hybridisation onto a flow cell. PCR was then used to enrich for adapter-containing cDNAs followed by quality control analysis of each sample library and quantification of the DNA library templates. Prior to sequencing, DNA templates were bridge-amplified to produce clonal clusters on the surface of the flow cell. Sequencing was then carried out using an Illumina HiSeq 2000. SRS376456 Control 6 RNA-Seq TRANSCRIPTOMIC unspecified 210 0 Application Read Forward 1 1 Application Read Reverse 106 Illumina HiSeq 2000 Illumina Pipeline (CASAVA) v1.8.2 SRX205684 Control 8 SRP016086 Poly(A)+ selection, mRNA library construction, cluster generation and sequencing was conducted by Macrogen Inc., Seoul, Korea. RNA samples were prepared for sequencing using a TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s instructions. mRNA was purified from total RNA by way of poly (A)+ selection before samples were fragmented and reverse transcribed to cDNA using random hexamer priming. Following fragment end-repair, individual frog cDNA libraries were tagged by ligation of unique indexing adapters to cDNA ends in preparation for hybridisation onto a flow cell. PCR was then used to enrich for adapter-containing cDNAs followed by quality control analysis of each sample library and quantification of the DNA library templates. Prior to sequencing, DNA templates were bridge-amplified to produce clonal clusters on the surface of the flow cell. Sequencing was then carried out using an Illumina HiSeq 2000. SRS376457 Control 8 RNA-Seq TRANSCRIPTOMIC unspecified 210 0 Application Read Forward 1 1 Application Read Reverse 106 Illumina HiSeq 2000 Illumina Pipeline (CASAVA) v1.8.2 SRX206002 Aestivator 5 SRP016086 Poly(A)+ selection, mRNA library construction, cluster generation and sequencing was conducted by Macrogen Inc., Seoul, Korea. RNA samples were prepared for sequencing using a TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s instructions. mRNA was purified from total RNA by way of poly (A)+ selection before samples were fragmented and reverse transcribed to cDNA using random hexamer priming. Following fragment end-repair, individual frog cDNA libraries were tagged by ligation of unique indexing adapters to cDNA ends in preparation for hybridisation onto a flow cell. PCR was then used to enrich for adapter-containing cDNAs followed by quality control analysis of each sample library and quantification of the DNA library templates. Prior to sequencing, DNA templates were bridge-amplified to produce clonal clusters on the surface of the flow cell. Sequencing was then carried out using an Illumina HiSeq 2000. SRS376714 Aestivator 5 RNA-Seq TRANSCRIPTOMIC unspecified 210 0 Application Read Forward 1 1 Application Read Reverse 106 Illumina HiSeq 2000 Illumina Pipeline (CASAVA) v1.8.2 SRX206003 Aestivator 8 SRP016086 Poly(A)+ selection, mRNA library construction, cluster generation and sequencing was conducted by Macrogen Inc., Seoul, Korea. RNA samples were prepared for sequencing using a TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s instructions. mRNA was purified from total RNA by way of poly (A)+ selection before samples were fragmented and reverse transcribed to cDNA using random hexamer priming. Following fragment end-repair, individual frog cDNA libraries were tagged by ligation of unique indexing adapters to cDNA ends in preparation for hybridisation onto a flow cell. PCR was then used to enrich for adapter-containing cDNAs followed by quality control analysis of each sample library and quantification of the DNA library templates. Prior to sequencing, DNA templates were bridge-amplified to produce clonal clusters on the surface of the flow cell. Sequencing was then carried out using an Illumina HiSeq 2000. SRS376715 Aestivator 8 RNA-Seq TRANSCRIPTOMIC unspecified 210 0 Application Read Forward 1 1 Application Read Reverse 106 Illumina HiSeq 2000 Illumina Pipeline (CASAVA) v1.8.2 SRX206004 Aestivator 9 SRP016086 Poly(A)+ selection, mRNA library construction, cluster generation and sequencing was conducted by Macrogen Inc., Seoul, Korea. RNA samples were prepared for sequencing using a TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s instructions. mRNA was purified from total RNA by way of poly (A)+ selection before samples were fragmented and reverse transcribed to cDNA using random hexamer priming. Following fragment end-repair, individual frog cDNA libraries were tagged by ligation of unique indexing adapters to cDNA ends in preparation for hybridisation onto a flow cell. PCR was then used to enrich for adapter-containing cDNAs followed by quality control analysis of each sample library and quantification of the DNA library templates. Prior to sequencing, DNA templates were bridge-amplified to produce clonal clusters on the surface of the flow cell. Sequencing was then carried out using an Illumina HiSeq 2000. SRS376716 Aestivator 9 RNA-Seq TRANSCRIPTOMIC unspecified 210 0 Application Read Forward 1 1 Application Read Reverse 106 Illumina HiSeq 2000 Illumina Pipeline (CASAVA) v1.8.2 SRX206005 Aestivator 10 SRP016086 Poly(A)+ selection, mRNA library construction, cluster generation and sequencing was conducted by Macrogen Inc., Seoul, Korea. RNA samples were prepared for sequencing using a TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s instructions. mRNA was purified from total RNA by way of poly (A)+ selection before samples were fragmented and reverse transcribed to cDNA using random hexamer priming. Following fragment end-repair, individual frog cDNA libraries were tagged by ligation of unique indexing adapters to cDNA ends in preparation for hybridisation onto a flow cell. PCR was then used to enrich for adapter-containing cDNAs followed by quality control analysis of each sample library and quantification of the DNA library templates. Prior to sequencing, DNA templates were bridge-amplified to produce clonal clusters on the surface of the flow cell. Sequencing was then carried out using an Illumina HiSeq 2000. SRS376453 Aestivator 10 RNA-Seq TRANSCRIPTOMIC unspecified 210 0 Application Read Forward 1 1 Application Read Reverse 106 Illumina HiSeq 2000 Illumina Pipeline (CASAVA) v1.8.2