SRX205678 PST0821 SRP017266 Genomic DNA was extracted for each race from dried urediniospores using the CTAB method. A single DNA library was prepared from 5 *g of total genomic DNA that was randomly fragmented using the Bioruptor sonicator (Diagenode). The library was prepared with the NEB Next DNA Sample Prep Master Mix according to manufacturer's instructions (New England Biolabs, MA USA). Fragments were purified using the Nucleotrap kit (Clontech) and eluted in 30 ul elution buffer and PCR-enriched using 14 PCR cycles. Paired end adapters and PCR primers were obtained from Illumina. Library quality and quantity were confirmed before sequencing using a Bioanalyzer (Agilent). SRS376451 PST_08/21 WGS GENOMIC unspecified 152 0 Application Read Forward 1 1 Application Read Reverse 77 Illumina HiSeq 2000 SRX220562 PST-08/21-2 SRP017266 Genomic DNA was extracted for each race from dried urediniospores using the CTAB method. A single DNA library was prepared from 5 *g of total genomic DNA that was randomly fragmented using the Bioruptor sonicator (Diagenode). The library was prepared with the NEB Next DNA Sample Prep Master Mix according to manufacturer's instructions (New England Biolabs, MA USA). Fragments were purified using the Nucleotrap kit (Clontech) and eluted in 30 ul elution buffer and PCR-enriched using 14 PCR cycles. Paired end adapters and PCR primers were obtained from Illumina. Primers with 6 nucleotide long barcodes were used. Library quality and quantity were confirmed before sequencing using a Bioanalyzer (Agilent). SRS376451 PST_08/21 WGS GENOMIC unspecified 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000