SRX247709 Pseudonitzschia microbiome SRP019069 DNA samples were extracted using RTL buffer with ß- mercaptoethanol, and lysed with sterile beads in a tissue lyser. The samples were then processed with the Qiagen DNA Stool Kit (Qiagen, Valencia, CA) and diluted to a final concentration of 20 ng/µl. Pyrosequencing was carried out by initially generating a sequence library from a one-step PCR of 30 cycles using a mixture of Hot Start and HotStar high fidelity Taq polymerase. The V6-V9 region of the 16S rDNA gene was targeted for the 454 pyrosequencing run using the universal primers Yellow939F and Yellow1492R. Tag-encoded FLX amplicon pyrosequencing analyses utilized Roche 454 FLX instrument with Titanium reagents, titanium procedures performed at the Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com). SRS399599 P. pungens-B2A AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Roche SFF file Tool QIIME 3.0 SRX247735 Pseudonitzschia pungens- C5A SRP019069 DNA samples were extracted using RTL buffer with ß- mercaptoethanol, and lysed with sterile beads in a tissue lyser. The samples were then processed with the Qiagen DNA Stool Kit (Qiagen, Valencia, CA) and diluted to a final concentration of 20 ng/µl. Pyrosequencing was carried out by initially generating a sequence library from a one-step PCR of 30 cycles using a mixture of Hot Start and HotStar high fidelity Taq polymerase. The V6-V9 region of the 16S rDNA gene was targeted for the 454 pyrosequencing run using the universal primers Yellow939F and Yellow1492R. Tag-encoded FLX amplicon pyrosequencing analyses utilized Roche 454 FLX instrument with Titanium reagents, titanium procedures performed at the Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com). SRS399609 P. pungens-C5A AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Roche SFFfile tool QIIME 3.0 SRX247736 Pseudonitzschia fraudelenta -1 SRP019069 DNA samples were extracted using RTL buffer with ß- mercaptoethanol, and lysed with sterile beads in a tissue lyser. The samples were then processed with the Qiagen DNA Stool Kit (Qiagen, Valencia, CA) and diluted to a final concentration of 20 ng/µl. Pyrosequencing was carried out by initially generating a sequence library from a one-step PCR of 30 cycles using a mixture of Hot Start and HotStar high fidelity Taq polymerase. The V6-V9 region of the 16S rDNA gene was targeted for the 454 pyrosequencing run using the universal primers Yellow939F and Yellow1492R. Tag-encoded FLX amplicon pyrosequencing analyses utilized Roche 454 FLX instrument with Titanium reagents, titanium procedures performed at the Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com). SRS399610 P. fraudelenta- 1 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Roche sfffile Tool QIIME 3.0 SRX247743 Pseudonitzschia fraudelenta - 8 SRP019069 DNA samples were extracted using RTL buffer with ß- mercaptoethanol, and lysed with sterile beads in a tissue lyser. The samples were then processed with the Qiagen DNA Stool Kit (Qiagen, Valencia, CA) and diluted to a final concentration of 20 ng/µl. Pyrosequencing was carried out by initially generating a sequence library from a one-step PCR of 30 cycles using a mixture of Hot Start and HotStar high fidelity Taq polymerase. The V6-V9 region of the 16S rDNA gene was targeted for the 454 pyrosequencing run using the universal primers Yellow939F and Yellow1492R. Tag-encoded FLX amplicon pyrosequencing analyses utilized Roche 454 FLX instrument with Titanium reagents, titanium procedures performed at the Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com). SRS399615 P. fraudelenta-8 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Roche SFF File tool QIIME 3.0 SRX247744 Pseudonitzschia australis-12 SRP019069 DNA samples were extracted using RTL buffer with ß- mercaptoethanol, and lysed with sterile beads in a tissue lyser. The samples were then processed with the Qiagen DNA Stool Kit (Qiagen, Valencia, CA) and diluted to a final concentration of 20 ng/µl. Pyrosequencing was carried out by initially generating a sequence library from a one-step PCR of 30 cycles using a mixture of Hot Start and HotStar high fidelity Taq polymerase. The V6-V9 region of the 16S rDNA gene was targeted for the 454 pyrosequencing run using the universal primers Yellow939F and Yellow1492R. Tag-encoded FLX amplicon pyrosequencing analyses utilized Roche 454 FLX instrument with Titanium reagents, titanium procedures performed at the Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com). SRS399616 P. australis- 12 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Roche ssffile tool QIIME 3.0 SRX247757 Pseudonitzschia australis -15 SRP019069 DNA samples were extracted using RTL buffer with ß- mercaptoethanol, and lysed with sterile beads in a tissue lyser. The samples were then processed with the Qiagen DNA Stool Kit (Qiagen, Valencia, CA) and diluted to a final concentration of 20 ng/µl. Pyrosequencing was carried out by initially generating a sequence library from a one-step PCR of 30 cycles using a mixture of Hot Start and HotStar high fidelity Taq polymerase. The V6-V9 region of the 16S rDNA gene was targeted for the 454 pyrosequencing run using the universal primers Yellow939F and Yellow1492R. Tag-encoded FLX amplicon pyrosequencing analyses utilized Roche 454 FLX instrument with Titanium reagents, titanium procedures performed at the Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com). SRS399629 P. australis-15 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Roche SFFfile Tool QIIME 3.0 SRX247761 Pseudonitzschia australis -B5 SRP019069 PRJNA182211 DNA samples were extracted using RTL buffer with ß- mercaptoethanol, and lysed with sterile beads in a tissue lyser. The samples were then processed with the Qiagen DNA Stool Kit (Qiagen, Valencia, CA) and diluted to a final concentration of 20 ng/µl. Pyrosequencing was carried out by initially generating a sequence library from a one-step PCR of 30 cycles using a mixture of Hot Start and HotStar high fidelity Taq polymerase. The V6-V9 region of the 16S rDNA gene was targeted for the 454 pyrosequencing run using the universal primers Yellow939F and Yellow1492R. Tag-encoded FLX amplicon pyrosequencing analyses utilized Roche 454 FLX instrument with Titanium reagents, titanium procedures performed at the Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com). SRS399632 SAMN01818674 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Roche SFF file tool QIIME 3.0