SRX366242 A08_0 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366243 A08_2 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366244 C2_0 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366245 C2_2 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366246 C3_0 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366247 C3_2 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366248 F412_0 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366249 F412_2 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366250 F414_0 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366251 F414_2 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366252 P01_0 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366253 P01_2 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366254 P03_0 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366255 P03_2 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366256 P07_0 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX366257 P07_2 SRP019932 PRJNA192558 Surface and bottom water samples of 8 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Bacterial universal primer 28F (5’-AGAGAGTTTGATCCTGGCTCAG-3’) 519R (5’-TTACCGCGGCTGCTGGCAC-3’) that spanned 16S rRNA gene V1–V3 region were used to amplify metagenomic DNA. SRS399191 PRE AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium