SRX256484 FM_P.leucopus_Female_Ovaries_RL3_Plate1_Region2 SRP020005 PRJNA192723 Livers, gonads and brains were immediately extracted from caught mice, rinsed with PBS, and immediately placed in RNALater* (Ambion Inc., Austin, TX) on ice before transport and storage at -80*C in the lab. Total RNA was extracted using RNaqueous* kits (Ambion, Austin, TX) or Trizol* reagent (Invitrogen, Carlsbad, CA). cDNA synthesis was carried out using the SMARTer™ cDNA synthesis kit (Clontech, Mountain View, CA) or the Roche cDNA synthesis kit (Roche Diagnostics, Indianapolis, IN). cDNA pools were sequenced with multiplex identifiers using standard 454 FLX Titanium protocols and sequenced on the 454 FLX platform. SRS405140 SAMN01991048 FM_P.leucopus_Female_Ovaries_RL3_Plate1_Region2 RNA-Seq TRANSCRIPTOMIC cDNA 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX+