SRX262164 Limulus polyphemus Joint Assembly and Mapping Lp-26 (offspring) SRP019763 PRJNA187356 libraries were constructed using genomic DNA extracted from whole limulus offspring. briefly, larvae were lysed and DNA extracted using disposable plastic pestles and eppendorf tubes followed by proteinase K lysis and DNA extraction as per Qiagen DNAeasy kit protocols. Genomic DNA was further cleaned using Zymo DNA clean columns. Illumina libraries were created using Nextera library prep kit as per kit protocols. Illumina libraries from offspring LP-11 through Lp-34 (all created using Nextera kit) were pooled in equimolar amounts (Lp-11-Lp-22, and Lp23-Lp-34 in two separate pools) for sequencing. Each pool in a separate flowcell lane. SRS409105 SAMN01901875 Lp-26 (offspring) WGS GENOMIC RANDOM 202 0 Application Read Forward 1 1 Application Read Reverse 102 Illumina HiSeq 2000