SRX266499 HMWDOM microcosm cDNA 27h SRP021115 PRJNA196863 Library preparation followed the Titanium Rapid Library Preparation protocol. In order to improve the retention of smaller cDNA molecules, adaptor-ligated libraries were not diluted prior to size selection with AMPure XP beads. Libraries were quantified using the Titanium Slingshot kit (Fluidigm) and added to emulsion PCR reactions at 0.1 molecules per bead. Sequencing and quality control were followed manufacturer recommendations. SRS413509 SAMN02045406 RNA-Seq METATRANSCRIPTOMIC cDNA 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium