SRX268450 GSM1125982_1 SRP021180 GSE46194 SRS414449 GSM1125982 ChIP-Seq GENOMIC ChIP 20 million cells were crosslinked for 10 minutes in 1% formaldehyde followed by 5 minutes of quenching in 0.125 M glycine. Cells were then washed with cold PBS pH7.4, and collected in the presence of cold cell lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, and Roche Complete Protease Inhibitor Cocktail Cat #11836145001). Nuclei were pelleted by centrifugation at 2,000 rpm for 5 minutes at 4C, and the supertanant discarded. Sheared chromatin was then prepared by sonicating the nuclei in the presence of RIPA buffer (1 x PBS pH 7.4, 1% NP-40, 0.5% NaDOC, Roche Protease Inhibitor Cocktail), followed by centrifugation to remove cell debris. To collected GRHL2-bound DNA, the chromatin was immunoprecipitated with an anti-GRHL2 antibody (Sigma HPA004820), the formaldehyde crosslinks were reversed by heating overnight at 65C, and the DNA was collected using Qiagen PCR cleanup columns. For input control samples, reverse-crosslinked DNA was isolated from an aliquot of the sonicated but not immunoprecipitated chromatin. DNA ends were blunted using a mixture of Klenow DNA polymerase, T4 DNA polymerase, and T4 polynucleotide kinase (PNK). Three-prime adenine was then added using 3'-5' exo- Klenow fragment, Illumina Y-adapters were ligated using T4 DNA ligase. The ligated DNA was gel size selected for 150-300 bp fragments, and PCR-amplified to make the final sequencing library. Samples were sequenced on an Illumina HiSeq 2000. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1125982 gds 301125982 GEO Accession GSM1125982 SRX268451 GSM1125983_1 SRP021180 GSE46194 SRS414450 GSM1125983 ChIP-Seq GENOMIC ChIP 20 million cells were crosslinked for 10 minutes in 1% formaldehyde followed by 5 minutes of quenching in 0.125 M glycine. Cells were then washed with cold PBS pH7.4, and collected in the presence of cold cell lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, and Roche Complete Protease Inhibitor Cocktail Cat #11836145001). Nuclei were pelleted by centrifugation at 2,000 rpm for 5 minutes at 4C, and the supertanant discarded. Sheared chromatin was then prepared by sonicating the nuclei in the presence of RIPA buffer (1 x PBS pH 7.4, 1% NP-40, 0.5% NaDOC, Roche Protease Inhibitor Cocktail), followed by centrifugation to remove cell debris. To collected GRHL2-bound DNA, the chromatin was immunoprecipitated with an anti-GRHL2 antibody (Sigma HPA004820), the formaldehyde crosslinks were reversed by heating overnight at 65C, and the DNA was collected using Qiagen PCR cleanup columns. For input control samples, reverse-crosslinked DNA was isolated from an aliquot of the sonicated but not immunoprecipitated chromatin. DNA ends were blunted using a mixture of Klenow DNA polymerase, T4 DNA polymerase, and T4 polynucleotide kinase (PNK). Three-prime adenine was then added using 3'-5' exo- Klenow fragment, Illumina Y-adapters were ligated using T4 DNA ligase. The ligated DNA was gel size selected for 150-300 bp fragments, and PCR-amplified to make the final sequencing library. Samples were sequenced on an Illumina HiSeq 2000. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1125983 gds 301125983 GEO Accession GSM1125983 SRX268452 GSM1125984_1 SRP021180 GSE46194 SRS414451 GSM1125984 ChIP-Seq GENOMIC ChIP 20 million cells were crosslinked for 10 minutes in 1% formaldehyde followed by 5 minutes of quenching in 0.125 M glycine. Cells were then washed with cold PBS pH7.4, and collected in the presence of cold cell lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, and Roche Complete Protease Inhibitor Cocktail Cat #11836145001). Nuclei were pelleted by centrifugation at 2,000 rpm for 5 minutes at 4C, and the supertanant discarded. Sheared chromatin was then prepared by sonicating the nuclei in the presence of RIPA buffer (1 x PBS pH 7.4, 1% NP-40, 0.5% NaDOC, Roche Protease Inhibitor Cocktail), followed by centrifugation to remove cell debris. To collected GRHL2-bound DNA, the chromatin was immunoprecipitated with an anti-GRHL2 antibody (Sigma HPA004820), the formaldehyde crosslinks were reversed by heating overnight at 65C, and the DNA was collected using Qiagen PCR cleanup columns. For input control samples, reverse-crosslinked DNA was isolated from an aliquot of the sonicated but not immunoprecipitated chromatin. DNA ends were blunted using a mixture of Klenow DNA polymerase, T4 DNA polymerase, and T4 polynucleotide kinase (PNK). Three-prime adenine was then added using 3'-5' exo- Klenow fragment, Illumina Y-adapters were ligated using T4 DNA ligase. The ligated DNA was gel size selected for 150-300 bp fragments, and PCR-amplified to make the final sequencing library. Samples were sequenced on an Illumina HiSeq 2000. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1125984 gds 301125984 GEO Accession GSM1125984 SRX268453 GSM1125985_1 SRP021180 GSE46194 SRS414452 GSM1125985 ChIP-Seq GENOMIC ChIP 20 million cells were crosslinked for 10 minutes in 1% formaldehyde followed by 5 minutes of quenching in 0.125 M glycine. Cells were then washed with cold PBS pH7.4, and collected in the presence of cold cell lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, and Roche Complete Protease Inhibitor Cocktail Cat #11836145001). Nuclei were pelleted by centrifugation at 2,000 rpm for 5 minutes at 4C, and the supertanant discarded. Sheared chromatin was then prepared by sonicating the nuclei in the presence of RIPA buffer (1 x PBS pH 7.4, 1% NP-40, 0.5% NaDOC, Roche Protease Inhibitor Cocktail), followed by centrifugation to remove cell debris. To collected GRHL2-bound DNA, the chromatin was immunoprecipitated with an anti-GRHL2 antibody (Sigma HPA004820), the formaldehyde crosslinks were reversed by heating overnight at 65C, and the DNA was collected using Qiagen PCR cleanup columns. For input control samples, reverse-crosslinked DNA was isolated from an aliquot of the sonicated but not immunoprecipitated chromatin. DNA ends were blunted using a mixture of Klenow DNA polymerase, T4 DNA polymerase, and T4 polynucleotide kinase (PNK). Three-prime adenine was then added using 3'-5' exo- Klenow fragment, Illumina Y-adapters were ligated using T4 DNA ligase. The ligated DNA was gel size selected for 150-300 bp fragments, and PCR-amplified to make the final sequencing library. Samples were sequenced on an Illumina HiSeq 2000. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1125985 gds 301125985 GEO Accession GSM1125985 SRX268454 GSM1125986_1 SRP021180 GSE46194 SRS414453 GSM1125986 ChIP-Seq GENOMIC ChIP 20 million cells were crosslinked for 10 minutes in 1% formaldehyde followed by 5 minutes of quenching in 0.125 M glycine. Cells were then washed with cold PBS pH7.4, and collected in the presence of cold cell lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, and Roche Complete Protease Inhibitor Cocktail Cat #11836145001). Nuclei were pelleted by centrifugation at 2,000 rpm for 5 minutes at 4C, and the supertanant discarded. Sheared chromatin was then prepared by sonicating the nuclei in the presence of RIPA buffer (1 x PBS pH 7.4, 1% NP-40, 0.5% NaDOC, Roche Protease Inhibitor Cocktail), followed by centrifugation to remove cell debris. To collected GRHL2-bound DNA, the chromatin was immunoprecipitated with an anti-GRHL2 antibody (Sigma HPA004820), the formaldehyde crosslinks were reversed by heating overnight at 65C, and the DNA was collected using Qiagen PCR cleanup columns. For input control samples, reverse-crosslinked DNA was isolated from an aliquot of the sonicated but not immunoprecipitated chromatin. DNA ends were blunted using a mixture of Klenow DNA polymerase, T4 DNA polymerase, and T4 polynucleotide kinase (PNK). Three-prime adenine was then added using 3'-5' exo- Klenow fragment, Illumina Y-adapters were ligated using T4 DNA ligase. The ligated DNA was gel size selected for 150-300 bp fragments, and PCR-amplified to make the final sequencing library. Samples were sequenced on an Illumina HiSeq 2000. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1125986 gds 301125986 GEO Accession GSM1125986 SRX268455 GSM1125987_1 SRP021180 GSE46194 SRS414454 GSM1125987 ChIP-Seq GENOMIC ChIP 20 million cells were crosslinked for 10 minutes in 1% formaldehyde followed by 5 minutes of quenching in 0.125 M glycine. Cells were then washed with cold PBS pH7.4, and collected in the presence of cold cell lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, and Roche Complete Protease Inhibitor Cocktail Cat #11836145001). Nuclei were pelleted by centrifugation at 2,000 rpm for 5 minutes at 4C, and the supertanant discarded. Sheared chromatin was then prepared by sonicating the nuclei in the presence of RIPA buffer (1 x PBS pH 7.4, 1% NP-40, 0.5% NaDOC, Roche Protease Inhibitor Cocktail), followed by centrifugation to remove cell debris. To collected GRHL2-bound DNA, the chromatin was immunoprecipitated with an anti-GRHL2 antibody (Sigma HPA004820), the formaldehyde crosslinks were reversed by heating overnight at 65C, and the DNA was collected using Qiagen PCR cleanup columns. For input control samples, reverse-crosslinked DNA was isolated from an aliquot of the sonicated but not immunoprecipitated chromatin. DNA ends were blunted using a mixture of Klenow DNA polymerase, T4 DNA polymerase, and T4 polynucleotide kinase (PNK). Three-prime adenine was then added using 3'-5' exo- Klenow fragment, Illumina Y-adapters were ligated using T4 DNA ligase. The ligated DNA was gel size selected for 150-300 bp fragments, and PCR-amplified to make the final sequencing library. Samples were sequenced on an Illumina HiSeq 2000. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1125987 gds 301125987 GEO Accession GSM1125987