SRX278555 GSM1138825_1 SRP022853 GSE46822 SRS421406 GSM1138825 ChIP-Seq GENOMIC ChIP For ChIP-chip experiments, 5 x 107 C6 glioma cells were cultured to 90% confluence. Cells were treated with 1% formaldehyde, and the cross-linked chromatin was sonicated (average size, 300 bp). Immunoprecipitation were performed with components of the ChIP IT kit according to the manufacturer’s instructions (Active Motif, Carlsbad, CA). Each sample was immunoprecipitated with 1 µg of anti-H3K4me3 antibody (Millipore). The amount and quality of immunoprecipitated DNA were determined by capillary electrophoresis with a Bioanalyzer 2100 and a High Sensitivity DNA LabChip kit from Agilent Technologies (Santa Clara, CA). Samples were amplified using the ChIP-Seq DNA sample prep kit from Illumina, following the manufacturer's instructions, and sequenced using Solid 5500xl platform (University of Uppsala Genomic Center). AB 5500xl Genetic Analyzer GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1138825 gds 301138825 GEO Accession GSM1138825 SRX278556 GSM1138826_1 SRP022853 GSE46822 SRS421407 GSM1138826 ChIP-Seq GENOMIC ChIP For ChIP-chip experiments, 5 x 107 C6 glioma cells were cultured to 90% confluence. Cells were treated with 1% formaldehyde, and the cross-linked chromatin was sonicated (average size, 300 bp). Immunoprecipitation were performed with components of the ChIP IT kit according to the manufacturer’s instructions (Active Motif, Carlsbad, CA). Each sample was immunoprecipitated with 1 µg of anti-H3K4me3 antibody (Millipore). The amount and quality of immunoprecipitated DNA were determined by capillary electrophoresis with a Bioanalyzer 2100 and a High Sensitivity DNA LabChip kit from Agilent Technologies (Santa Clara, CA). Samples were amplified using the ChIP-Seq DNA sample prep kit from Illumina, following the manufacturer's instructions, and sequenced using Solid 5500xl platform (University of Uppsala Genomic Center). AB 5500xl Genetic Analyzer GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1138826 gds 301138826 GEO Accession GSM1138826