SRX305010 GSM1161947 SRP025994 GSE47885 SRS444334 GSM1161947 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from C57BL/6 RAG-/- pro-B cells using Trizol® (Life Technologies Corp., Carlsbad CA) and genomic DNA was eliminated using the genomic DNA wipeout buffer in the QuantiTect Reverse transcription kit (QIAGEN). A final purification of the RNA was performed with the RNeasy kit from QIAGEN. For each sample, 100 ng of total RNA was used to make RNASeq libraries using the NuGEN Encore Complete DR kits following manufacturer's recommended protocols. Sequencing libraries were gel purified to ensure insert sizes were larger than 100 bp in length and sequenced on an Ilumina HiSeq2000 for 100 bases plus 7 bases for indexing. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1161947 gds 301161947 GEO Accession GSM1161947 SRX305011 GSM1161948 SRP025994 GSE47885 SRS444335 GSM1161948 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from C57BL/6 RAG-/- pro-B cells using Trizol® (Life Technologies Corp., Carlsbad CA) and genomic DNA was eliminated using the genomic DNA wipeout buffer in the QuantiTect Reverse transcription kit (QIAGEN). A final purification of the RNA was performed with the RNeasy kit from QIAGEN. For each sample, 100 ng of total RNA was used to make RNASeq libraries using the NuGEN Encore Complete DR kits following manufacturer's recommended protocols. Sequencing libraries were gel purified to ensure insert sizes were larger than 100 bp in length and sequenced on an Ilumina HiSeq2000 for 100 bases plus 7 bases for indexing. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1161948 gds 301161948 GEO Accession GSM1161948