SRX305384 GSM1162717 SRP026013 GSE47933 SRS444561 GSM1162717 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina Genome Analyzer IIx GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162717 gds 301162717 GEO Accession GSM1162717 SRX305385 GSM1162718 SRP026013 GSE47933 SRS444539 GSM1162718 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina Genome Analyzer IIx GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162718 gds 301162718 GEO Accession GSM1162718 SRX305386 GSM1162719 SRP026013 GSE47933 SRS444540 GSM1162719 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina Genome Analyzer IIx GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162719 gds 301162719 GEO Accession GSM1162719 SRX305387 GSM1162720 SRP026013 GSE47933 SRS444541 GSM1162720 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina Genome Analyzer IIx GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162720 gds 301162720 GEO Accession GSM1162720 SRX305388 GSM1162721 SRP026013 GSE47933 SRS444542 GSM1162721 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina Genome Analyzer IIx GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162721 gds 301162721 GEO Accession GSM1162721 SRX305389 GSM1162722 SRP026013 GSE47933 SRS444543 GSM1162722 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina Genome Analyzer IIx GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162722 gds 301162722 GEO Accession GSM1162722 SRX305390 GSM1162723 SRP026013 GSE47933 SRS444544 GSM1162723 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina Genome Analyzer IIx GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162723 gds 301162723 GEO Accession GSM1162723 SRX305391 GSM1162724 SRP026013 GSE47933 SRS444545 GSM1162724 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina Genome Analyzer IIx GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162724 gds 301162724 GEO Accession GSM1162724 SRX305392 GSM1162725 SRP026013 GSE47933 SRS444546 GSM1162725 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162725 gds 301162725 GEO Accession GSM1162725 SRX305393 GSM1162726 SRP026013 GSE47933 SRS444547 GSM1162726 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162726 gds 301162726 GEO Accession GSM1162726 SRX305394 GSM1162727 SRP026013 GSE47933 SRS444548 GSM1162727 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162727 gds 301162727 GEO Accession GSM1162727 SRX305395 GSM1162728 SRP026013 GSE47933 SRS444551 GSM1162728 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162728 gds 301162728 GEO Accession GSM1162728 SRX305396 GSM1162729 SRP026013 GSE47933 SRS444549 GSM1162729 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162729 gds 301162729 GEO Accession GSM1162729 SRX305397 GSM1162730 SRP026013 GSE47933 SRS444550 GSM1162730 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162730 gds 301162730 GEO Accession GSM1162730 SRX305398 GSM1162731 SRP026013 GSE47933 SRS444553 GSM1162731 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162731 gds 301162731 GEO Accession GSM1162731 SRX305399 GSM1162732 SRP026013 GSE47933 SRS444552 GSM1162732 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300. Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1162732 gds 301162732 GEO Accession GSM1162732