SRX315128 GSM1174375 SRP026329 GSE48299 SRS452602 GSM1174375 OTHER OTHER other Based on Pan et al. 2011 Cell 144:719-731. Whole cell extracts were prepared by grinding frozen cells in a bead mill and resuspending powder in 10%TCA. Pellets were then extracted with SDS and soluble protein was subjected to affinity purification of Spo11-protein A fusion with IgG sepharose beads. Protein was digested with proteinase K and Spo11-associated oligonucleotides were recovered by ethanol precipitation. Based on Pan et al. 2011 Cell 144:719-731. Oligonucleotides were tailed with ribo-G using rGTP and terminal transferase, then ligated to duplex DNA adaptors with a dC overhang using T4 RNA ligase 2. Complementary strands were synthesized with Klenow, then purified by denaturing polyacrylamide electrophoresis. Purified complementary strands were subjected to 3' tailing with ribo-G using rGTP and terminal transferase, then another duplex adaptor was ligated using T4 RNA ligase 2. Ligated material was amplified with 16 cycles of PCR to add Illumina HiSeq adaptors Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1174375 gds 301174375 GEO Accession GSM1174375 SRX315129 GSM1174376 SRP026329 GSE48299 SRS452603 GSM1174376 OTHER OTHER other Based on Pan et al. 2011 Cell 144:719-731. Whole cell extracts were prepared by grinding frozen cells in a bead mill and resuspending powder in 10%TCA. Pellets were then extracted with SDS and soluble protein was subjected to affinity purification of Spo11-protein A fusion with IgG sepharose beads. Protein was digested with proteinase K and Spo11-associated oligonucleotides were recovered by ethanol precipitation. Based on Pan et al. 2011 Cell 144:719-731. Oligonucleotides were tailed with ribo-G using rGTP and terminal transferase, then ligated to duplex DNA adaptors with a dC overhang using T4 RNA ligase 2. Complementary strands were synthesized with Klenow, then purified by denaturing polyacrylamide electrophoresis. Purified complementary strands were subjected to 3' tailing with ribo-G using rGTP and terminal transferase, then another duplex adaptor was ligated using T4 RNA ligase 2. Ligated material was amplified with 16 cycles of PCR to add Illumina HiSeq adaptors Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1174376 gds 301174376 GEO Accession GSM1174376 SRX315130 GSM1174377 SRP026329 GSE48299 SRS452604 GSM1174377 OTHER OTHER other Based on Pan et al. 2011 Cell 144:719-731. Whole cell extracts were prepared by grinding frozen cells in a bead mill and resuspending powder in 10%TCA. Pellets were then extracted with SDS and soluble protein was subjected to affinity purification of Spo11-protein A fusion with IgG sepharose beads. Protein was digested with proteinase K and Spo11-associated oligonucleotides were recovered by ethanol precipitation. Based on Pan et al. 2011 Cell 144:719-731. Oligonucleotides were tailed with ribo-G using rGTP and terminal transferase, then ligated to duplex DNA adaptors with a dC overhang using T4 RNA ligase 2. Complementary strands were synthesized with Klenow, then purified by denaturing polyacrylamide electrophoresis. Purified complementary strands were subjected to 3' tailing with ribo-G using rGTP and terminal transferase, then another duplex adaptor was ligated using T4 RNA ligase 2. Ligated material was amplified with 16 cycles of PCR to add Illumina HiSeq adaptors Illumina HiSeq 2000 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1174377 gds 301174377 GEO Accession GSM1174377 SRX315131 GSM1174378 SRP026329 GSE48299 SRS452605 GSM1174378 OTHER OTHER other Based on Pan et al. 2011 Cell 144:719-731. Whole cell extracts were prepared by grinding frozen cells in a bead mill and resuspending powder in 10%TCA. Pellets were then extracted with SDS and soluble protein was subjected to affinity purification of Spo11-protein A fusion with IgG sepharose beads. Protein was digested with proteinase K and Spo11-associated oligonucleotides were recovered by ethanol precipitation. Based on Pan et al. 2011 Cell 144:719-731. Oligonucleotides were tailed with ribo-G using rGTP and terminal transferase, then ligated to duplex DNA adaptors with a dC overhang using T4 RNA ligase 2. Complementary strands were synthesized with Klenow, then purified by denaturing polyacrylamide electrophoresis. Purified complementary strands were subjected to 3' tailing with ribo-G using rGTP and terminal transferase, then another duplex adaptor was ligated using T4 RNA ligase 2. Ligated material was amplified with 16 cycles of PCR to add Illumina HiSeq adaptors Illumina HiSeq 2500 GEO Sample http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1174378 gds 301174378 GEO Accession GSM1174378