SRX320099 Oreochromis niloticus RNA-seq SRP026706 The Illunima HiSeqTM2000 technology was applied to determine the transcriptome sequence of Oreochromis niloticus spleen tissue and a comparative analysis of transcriptome date between the control and the inactivity vaccine of Streptococcus agalactiae ZQ0910 infected group was performed in this study. The cDNA library was constructed according to the manufacturer’s instructions (Illumina/Hiseq-2000 RNA-seq). The mRNA molecules containing poly (A) were purified using Sera-mag Magnetic Oligo (dT) Beads from the RNA samples.A fragmentation buffer was added to break the mRNA into short fragments. Using these short fragments as templates, the first strand of cDNA was synthesized.The second strand of cDNA was synthesized using the buffers containing dNTPs, RNaseH, and DNA polymerase I. The synthesized cDNA was purified using the QiaQuick PCR extraction kit (Qiagen). The purified cDNA was connected with the sequencing adapters. A range of cDNA fragments (200*25 bp) were excised from 2% TAE-agarose gel (Certified Low-Range Ultra Agarose, Biorad) using QIAquick Gel Extraction Kit(Qiagen). The library was sequenced using the Illumina/Hiseq-2000 RNA-seq. SRS456494 GIFT-S-Sa RNA-Seq TRANSCRIPTOMIC RANDOM 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000 SRX556513 Oreochromis niloticus after S. agalactiae infection SRP026706 The Illunima HiSeqTM2000 technology was applied to determine the transcriptome sequence of Oreochromis niloticus spleen tissue and a comparative analysis of transcriptome date between the control and the inactivity vaccine of Streptococcus agalactiae ZQ0910 infected group was performed in this study. The cDNA library was constructed according to the manufacturer’s instructions (Illumina/Hiseq-2000 RNA-seq). The mRNA molecules containing poly (A) were purified using Sera-mag Magnetic Oligo (dT) Beads from the RNA samples.A fragmentation buffer was added to break the mRNA into short fragments. Using these short fragments as templates, the first strand of cDNA was synthesized.The second strand of cDNA was synthesized using the buffers containing dNTPs, RNaseH, and DNA polymerase I. The synthesized cDNA was purified using the QiaQuick PCR extraction kit (Qiagen). The purified cDNA was connected with the sequencing adapters. A range of cDNA fragments (200*25 bp) were excised from 2% TAE-agarose gel (Certified Low-Range Ultra Agarose, Biorad) using QIAquick Gel Extraction Kit(Qiagen). The library was sequenced using the Illumina/Hiseq-2000 RNA-seq. SRS623518 GIFT-S-Sa RNA-Seq TRANSCRIPTOMIC RANDOM 200 0 Application Read Forward 1 1 Application Read Reverse 101 Illumina HiSeq 2000