SRX7255709 GSM4200358 SRP234430 SRS5753247 GSM4200358 RNA-Seq TRANSCRIPTOMIC cDNA For each group skin tissues were obtained from at least 3 independent female, prior to digestion. To prepare dorsal skin single cell suspension for single-cell RNA sequencing, the back skin tissues were isolated via microdissection and 0.25 % trypsin/EDTA solution was used to digest dorsal skin tissues at 37 °C for 30 min，mechanically dissociate the tissue once every 10 min. 1 mg/ml collagenase IV (Sigma, St Louis, MO, USA) was used to digest the tissue which digested by trypsin at 37 °C for 30 min. the skin tissues were mechanically dissociated into single cell suspension by pipetting, the cell suspensions were then filtered through a 30 μm nylon cell strainer, to remove villus debris(BD Falcon, BD Biosciences, San Jose, CA, USA) prior to single cell library construction. Single-cell RNA-seq libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10x Genomics Inc., Pleasanton, CA, USA, Cat#120237) following the manufacture's introduction. Illumina HiSeq 2000 gds 304200358 GSM4200358 GEO Accession GSM4200358 SRX7255710 GSM4200359 SRP234430 SRS5753248 GSM4200359 RNA-Seq TRANSCRIPTOMIC cDNA For each group skin tissues were obtained from at least 3 independent female, prior to digestion. To prepare dorsal skin single cell suspension for single-cell RNA sequencing, the back skin tissues were isolated via microdissection and 0.25 % trypsin/EDTA solution was used to digest dorsal skin tissues at 37 °C for 30 min，mechanically dissociate the tissue once every 10 min. 1 mg/ml collagenase IV (Sigma, St Louis, MO, USA) was used to digest the tissue which digested by trypsin at 37 °C for 30 min. the skin tissues were mechanically dissociated into single cell suspension by pipetting, the cell suspensions were then filtered through a 30 μm nylon cell strainer, to remove villus debris(BD Falcon, BD Biosciences, San Jose, CA, USA) prior to single cell library construction. Single-cell RNA-seq libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10x Genomics Inc., Pleasanton, CA, USA, Cat#120237) following the manufacture's introduction. Illumina HiSeq 2000 gds 304200359 GSM4200359 GEO Accession GSM4200359 SRX7255711 GSM4200360 SRP234430 SRS5753249 GSM4200360 RNA-Seq TRANSCRIPTOMIC cDNA For each group skin tissues were obtained from at least 3 independent female, prior to digestion. To prepare dorsal skin single cell suspension for single-cell RNA sequencing, the back skin tissues were isolated via microdissection and 0.25 % trypsin/EDTA solution was used to digest dorsal skin tissues at 37 °C for 30 min，mechanically dissociate the tissue once every 10 min. 1 mg/ml collagenase IV (Sigma, St Louis, MO, USA) was used to digest the tissue which digested by trypsin at 37 °C for 30 min. the skin tissues were mechanically dissociated into single cell suspension by pipetting, the cell suspensions were then filtered through a 30 μm nylon cell strainer, to remove villus debris(BD Falcon, BD Biosciences, San Jose, CA, USA) prior to single cell library construction. Single-cell RNA-seq libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10x Genomics Inc., Pleasanton, CA, USA, Cat#120237) following the manufacture's introduction. Illumina HiSeq 2000 gds 304200360 GSM4200360 GEO Accession GSM4200360