SRS6220532 SAMN14214399 4932 Saccharomyces cerevisiae Transformed library unsorted fraction bioproject 608939 PRJNA608939 strain ROY5634 (library) isolation_source ROY5634 collection_date not collected geo_loc_name not applicable sample_type cell culture genotype MATa ADE2 lys2D leu2-3,112 his3-11 ura3-1 trp1-1 can1-100 rpl18b::BFP2-2 dev_stage OD600 described in growth protocol tissue liquid culture growth_protocol 5ml of yeast cells containing the library were used to inoculate 50ml YMD medium containing adenine, leucine, lysine, histidine and tryptophan (lacking uracil) and cells were grown at room temperature overnight (approximately 3 doublings). This culture was then used to inoculate 250ml YMD-uracil and grown at 30C for 3h. Cells were pelleted and resuspended into 250ul 1xPBS and 1%BSA at a concentration of 1 OD/ml, filtered through a Nitex mesh and sorted. treatment unsorted BioSampleModel Microbe, viral or environmental SRS6220533 SAMN14214400 4932 Saccharomyces cerevisiae Transformed library sorted fraction: barcoded library by bin. NB01-NB04 bioproject 608939 PRJNA608939 strain ROY5634 (library) isolation_source ROY5634 collection_date not collected geo_loc_name not applicable sample_type FACS genotype MATa ADE2 lys2D leu2-3,112 his3-11 ura3-1 trp1-1 can1-100 rpl18b::BFP2-2 dev_stage OD600 described in growth protocol tissue liquid culture growth_protocol 5ml of yeast cells containing the library were used to inoculate 50ml YMD medium containing adenine, leucine, lysine, histidine and tryptophan (lacking uracil) and cells were grown at room temperature overnight (approximately 3 doublings). This culture was then used to inoculate 250ml YMD-uracil and grown at 30C for 3h. Cells were pelleted and resuspended into 250ul 1xPBS and 1%BSA at a concentration of 1 OD/ml, filtered through a Nitex mesh and sorted. treatment sorted BioSampleModel Microbe, viral or environmental