SRX370991 CCQM-NMIA-454-Ecoli SRP032371 Three subregions of the 16S rRNA gene were PCR amplified from IRMM-449 Escherichia coli O157:H7 EDL933 and IRMM-447 Listeria monocytogenes str 4b NCTC 11994 reference materialreference material and sequenced using the following primer pairs 27F:534R, 357F:926R, and 986F:1492R. Each amplicon was sequenced in both directions. Target Primer name Combined sequence primer= Primer A or B, Key, MID, template specific 5' to 3' E. coli UHTS1 E-27F-fusion cgtatcgcctccctcgcgccatcagacgagtgcgtagagtttgatcAtggctcag UHTS1 E-534R-fusion ctatgcgccttgccagcccgctcagacgctcgacaattaccgcggctgctgg UHTS2 E-357F-fusion cgtatcgcctccctcgcgccatcagagacgcactccctacgggaggcagcag UHTS2 E-926R-fusion ctatgcgccttgccagcccgctcagagcactgtagccgtcaattcAtttGagt UHTS3 E-U968F-fusion cgtatcgcctccctcgcgccatcagatcagacacgaacgcgaagaaccttac UHTS3 E-1492R-fusion ctatgcgccttgccagcccgctcagatatcgcgagtacggTtaccttgttaCgactt L. monocytogenes UHTS1 L-27F-fusion cgtatcgcctccctcgcgccatcagcgtgtctctaagagtttgatcctggctcag UHTS1 L-534R-fusion ctatgcgccttgccagcccgctcagctcgcgtgtcattaccgcggctgctgg UHTS2 L-357F-fusion cgtatcgcctccctcgcgccatcagtagtatcagccctacgggaggcagcag UHTS2 L-926R-fusion ctatgcgccttgccagcccgctcagtctctatgcgccgtcaattcCtttGagt UHTS3 L-U968F-fusion cgtatcgcctccctcgcgccatcagtgatacgtctaacgcgaagaaccttac UHTS3 L-1492R-fusion ctatgcgccttgccagcccgctcagtactgagctatacggCtaccttgttaCgactt SRS497312 ecoli_nmia_454 AMPLICON GENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS Junior