SRX371501 GSM1253183 SRP032422 GSE51816 SRS497006 GSM1253183 ChIP-Seq GENOMIC ChIP Cells were fixed in 1% formaldehyde for 10 min and cross-linking was quenched by addition of 0.125 M glycine. Cells were then harvested in ChIP buffer and sonicated with a Bioruptor UCD-300 to obtain fragments around 250 bp in size. Plolyclonal custom-made PRDM5 antibodies were used for Immunoprecipitation. Precipitated DNA was recovered by purification with a QIAquick PCR purification kit (Qiagen) Libraries for sequencing were obtained using a ChIP-Seq DNA sample prep kit (Illumina) according to the manufacturer's reccomendations Illumina Genome Analyzer II GEO Sample GSM1253183 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1253183 gds 301253183 GEO Accession GSM1253183 SRX371502 GSM1253184 SRP032422 GSE51816 SRS497007 GSM1253184 ChIP-Seq GENOMIC ChIP Cells were fixed in 1% formaldehyde for 10 min and cross-linking was quenched by addition of 0.125 M glycine. Cells were then harvested in ChIP buffer and sonicated with a Bioruptor UCD-300 to obtain fragments around 250 bp in size. Plolyclonal custom-made PRDM5 antibodies were used for Immunoprecipitation. Precipitated DNA was recovered by purification with a QIAquick PCR purification kit (Qiagen) Libraries for sequencing were obtained using a ChIP-Seq DNA sample prep kit (Illumina) according to the manufacturer's reccomendations Illumina Genome Analyzer II GEO Sample GSM1253184 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1253184 gds 301253184 GEO Accession GSM1253184 SRX371503 GSM1253185 SRP032422 GSE51816 SRS497008 GSM1253185 ChIP-Seq GENOMIC ChIP Cells were fixed in 1% formaldehyde for 10 min and cross-linking was quenched by addition of 0.125 M glycine. Cells were then harvested in ChIP buffer and sonicated with a Bioruptor UCD-300 to obtain fragments around 250 bp in size. Plolyclonal custom-made PRDM5 antibodies were used for Immunoprecipitation. Precipitated DNA was recovered by purification with a QIAquick PCR purification kit (Qiagen) Libraries for sequencing were obtained using a ChIP-Seq DNA sample prep kit (Illumina) according to the manufacturer's reccomendations Illumina Genome Analyzer II GEO Sample GSM1253185 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1253185 gds 301253185 GEO Accession GSM1253185 SRX371504 GSM1253186 SRP032422 GSE51816 SRS497009 GSM1253186 ChIP-Seq GENOMIC ChIP Cells were fixed in 1% formaldehyde for 10 min and cross-linking was quenched by addition of 0.125 M glycine. Cells were then harvested in ChIP buffer and sonicated with a Bioruptor UCD-300 to obtain fragments around 250 bp in size. Plolyclonal custom-made PRDM5 antibodies were used for Immunoprecipitation. Precipitated DNA was recovered by purification with a QIAquick PCR purification kit (Qiagen) Libraries for sequencing were obtained using a ChIP-Seq DNA sample prep kit (Illumina) according to the manufacturer's reccomendations Illumina Genome Analyzer II GEO Sample GSM1253186 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1253186 gds 301253186 GEO Accession GSM1253186