SRX8846622 GSM4703698 SRP274175 SRS7109998 GSM4703698 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703698 GSM4703698 GEO Accession GSM4703698 SRX8846623 GSM4703699 SRP274175 SRS7109999 GSM4703699 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703699 GSM4703699 GEO Accession GSM4703699 SRX8846624 GSM4703700 SRP274175 SRS7110000 GSM4703700 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703700 GSM4703700 GEO Accession GSM4703700 SRX8846625 GSM4703701 SRP274175 SRS7110001 GSM4703701 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703701 GSM4703701 GEO Accession GSM4703701 SRX8846626 GSM4703702 SRP274175 SRS7110002 GSM4703702 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703702 GSM4703702 GEO Accession GSM4703702 SRX8846627 GSM4703703 SRP274175 SRS7110003 GSM4703703 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703703 GSM4703703 GEO Accession GSM4703703 SRX8846628 GSM4703704 SRP274175 SRS7110005 GSM4703704 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703704 GSM4703704 GEO Accession GSM4703704 SRX8846629 GSM4703705 SRP274175 SRS7110004 GSM4703705 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703705 GSM4703705 GEO Accession GSM4703705 SRX8846630 GSM4703706 SRP274175 SRS7110006 GSM4703706 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703706 GSM4703706 GEO Accession GSM4703706 SRX8846631 GSM4703707 SRP274175 SRS7110007 GSM4703707 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703707 GSM4703707 GEO Accession GSM4703707 SRX8846632 GSM4703708 SRP274175 SRS7110008 GSM4703708 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703708 GSM4703708 GEO Accession GSM4703708 SRX8846633 GSM4703709 SRP274175 SRS7110009 GSM4703709 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703709 GSM4703709 GEO Accession GSM4703709 SRX8846634 GSM4703710 SRP274175 SRS7110011 GSM4703710 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703710 GSM4703710 GEO Accession GSM4703710 SRX8846635 GSM4703711 SRP274175 SRS7110010 GSM4703711 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703711 GSM4703711 GEO Accession GSM4703711 SRX8846636 GSM4703712 SRP274175 SRS7110012 GSM4703712 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Illumina HiSeq 2000 gds 304703712 GSM4703712 GEO Accession GSM4703712 SRX8846637 GSM4703713 SRP274175 SRS7110013 GSM4703713 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703713 GSM4703713 GEO Accession GSM4703713 SRX8846638 GSM4703714 SRP274175 SRS7110014 GSM4703714 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703714 GSM4703714 GEO Accession GSM4703714 SRX8846639 GSM4703715 SRP274175 SRS7110015 GSM4703715 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703715 GSM4703715 GEO Accession GSM4703715 SRX8846640 GSM4703716 SRP274175 SRS7110016 GSM4703716 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703716 GSM4703716 GEO Accession GSM4703716 SRX8846641 GSM4703717 SRP274175 SRS7110017 GSM4703717 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703717 GSM4703717 GEO Accession GSM4703717 SRX8846642 GSM4703718 SRP274175 SRS7110018 GSM4703718 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703718 GSM4703718 GEO Accession GSM4703718 SRX8846643 GSM4703719 SRP274175 SRS7110019 GSM4703719 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703719 GSM4703719 GEO Accession GSM4703719 SRX8846644 GSM4703720 SRP274175 SRS7110020 GSM4703720 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703720 GSM4703720 GEO Accession GSM4703720 SRX8846645 GSM4703721 SRP274175 SRS7110021 GSM4703721 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703721 GSM4703721 GEO Accession GSM4703721 SRX8846646 GSM4703722 SRP274175 SRS7110023 GSM4703722 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703722 GSM4703722 GEO Accession GSM4703722 SRX8846647 GSM4703723 SRP274175 SRS7110022 GSM4703723 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703723 GSM4703723 GEO Accession GSM4703723 SRX8846648 GSM4703724 SRP274175 SRS7110024 GSM4703724 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703724 GSM4703724 GEO Accession GSM4703724 SRX8846649 GSM4703725 SRP274175 SRS7110025 GSM4703725 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703725 GSM4703725 GEO Accession GSM4703725 SRX8846650 GSM4703726 SRP274175 SRS7110026 GSM4703726 RNA-Seq TRANSCRIPTOMIC cDNA Illumina: Total RNA samples were prepared for sequencing using the Qiagen Allprep total RNA kit (Qiagen). Briefly, tissue sections (20-30µg) taken from the middle of the kidneys were lyzed and homogenized in 600 µl Buffer RLT in 2 mL Magna Lyser green beads tubes (Roche Life sciences, Norway) at 600 rpm for 30 sec using the Precellys 24 tissue homogenizer (Bertin Thechnologies, France). The lysates were centrifuged at full speed for 3 min, the supernatant was carefully transferred to the AllPrep spin column, and RNA isolation was performed according to the manufacturer's protocol. The Isolated RNA were analyzed by Agilent. Only RNA samples of high quality (RNA Integrity number (RIN) ≥8.0) were used. Ion Torrent: Total RNA of lymph nodes of four and eight weeks, proteinuric mice, immune aggregates, dissected manually under the loupe (magnification:1:10) of protein uric kidney and kidney tissue from the same kidney purified by TRIzol regent (Ambion, Thermo Fisher, Oslo, Norway) according to manufacturer's instructions. Total RNA of lymph nodes from anti-dsDNA antibody positive mice were isolated using miRNeasy mini kit (Qiagen, Oslo, Norway). The concentration and quality of extracted total RNA were assessed using the AgilentR RNA 6000 nano kit with the AgilentR 2100 BioanalyzerR instrument (Agilent, Matriks AS, Norway). All RNA used in this study had RNA integrity value (RIN value) ≥ 7. Illumina: RNA libraries were prepared for sequencing using standard Illumina protocols Ion Torrent: Polyadenylated (poly(A)) mRNA was isolated from total RNA using Dynabeads mRNA Direct Micro Kit (Ambion, Thermo Fisher) followed by its fragmentation using RNase III with an average sizes of 100-200 nt. The fragmented mRNA was cleaned up and determined the yield and size distribution using the AgilentR RNA 6000 Pico kit with the AgilentR 2100 BioanalyzerR instrument. After adapter ligation at both ends of fragmented mRNA, performed reverse transcription, followed by PCR amplification. After purification of amplified cDNA analyzed to assess the yield and size distribution using AgilentR High Sensitivity DNS kit with AgilentR 2100 BioanalyzerR instrument. Ion Torrent PGM gds 304703726 GSM4703726 GEO Accession GSM4703726