SRX8868839 far_1_24_10 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129729 far_1_24_10 far_1_24_10 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868840 far_2_2_8 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129730 far_2_2_8 far_2_2_8 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868841 mid_3_2_8 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129731 mid_3_2_8 mid_3_2_8 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868842 near_1_2_8 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129732 near_1_2_8 near_1_2_8 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868843 near_1_24_10 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129733 near_1_24_10 near_1_24_10 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868844 near_2_2_8 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129734 near_2_2_8 near_2_2_8 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868845 near_2_24_10 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129735 near_2_24_10 near_2_24_10 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868846 near_3_2_8 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129736 near_3_2_8 near_3_2_8 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868847 near_3_24_10 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129737 near_3_24_10 near_3_24_10 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868848 far_2_24_10 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129738 far_2_24_10 far_2_24_10 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868849 far_3_2_8 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129739 far_3_2_8 far_3_2_8 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868850 far_3_24_10 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129740 far_3_24_10 far_3_24_10 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868851 mid_1_2_8 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129741 mid_1_2_8 mid_1_2_8 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868852 mid_1_24_10 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129742 mid_1_24_10 mid_1_24_10 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868853 mid_2_2_8 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129743 mid_2_2_8 mid_2_2_8 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868854 mid_2_24_10 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129744 mid_2_24_10 mid_2_24_10 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500 SRX8868855 mid_3_24_10 SRP275575 bp0 Water samples were filtered through a 5 m, 47-mm Nuclepore filter, and immediately flash frozen in liquid nitrogen. To extract RNA, cells were homogenized in a custom guanadidium thiocyanate buffer in a bead beater using MoBio lysing matrix E and vortexed for 15 s, then RNA was extracted from the lysate using a TRIzol procedure followed by a Qiagen RNeasy column clean up. RNA was checked for quality using a Fisher nanodrop (minimum quantity = 25 ng RNA per l), and quantity using an Agilent bioanalyzer with a minimum RIN of 7 (lowered to accommodate the multiple taxa within the extraction and the low yield). Samples were prepared for sequencing at the Ramaciotti Centre for gene function analysis using Illuminas TruSeq stranded mRNA kit with extended PCR to compensate for the low RNA yield. Samples were run on Illuminas NextSeq 500 1X75bp using a high output protocol. SRS7129745 mid_3_24_10 mid_3_24_10 RNA-Seq METATRANSCRIPTOMIC PolyA NextSeq 500