SRX377754 IP Microbiota SRP033019 PRJNA227795 PCR amplicon libraries of the V5-V6 region of 16S rRNA genes were prepared from metagenomic DNA. Universal primers U789f (5’-TAGATACCCSSGTAGTCC-3’) and U1068r (5’-CTGACGRCRGCCATGC-3’), targeting both bacteria and archaea, were adapted for pyrosequencing by the addition of sequencing adapters and multiplex identifier (MID) sequences. To minimise PCR bias three individual reactions were performed per template and equimolar amounts of PCR products from each of the three reactions were pooled for pyrosequencing. PCR products were purified using Qiagen PCR Purification Kit (Qiagen Ltd., UK) as per the manufacturer’s instructions. Barcoded samples were pooled and sequenced on a GS FLX Titanium platform. SRS502558 SAMN02402455 BD92 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX377938 PC_Microbiota SRP033019 PCR amplicon libraries of the V5-V6 region of 16S rRNA genes were prepared from metagenomic DNA. Universal primers U789f (5’-TAGATACCCSSGTAGTCC-3’) and U1068r (5’-CTGACGRCRGCCATGC-3’), targeting both bacteria and archaea, were adapted for pyrosequencing by the addition of sequencing adapters and multiplex identifier (MID) sequences. To minimise PCR bias three individual reactions were performed per template and equimolar amounts of PCR products from each of the three reactions were pooled for pyrosequencing. PCR products were purified using Qiagen PCR Purification Kit (Qiagen Ltd., UK) as per the manufacturer’s instructions. Barcoded samples were pooled and sequenced on a GS FLX Titanium platform. SRS502738 BD130 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX377939 LD_Microbiota SRP033019 PCR amplicon libraries of the V5-V6 region of 16S rRNA genes were prepared from metagenomic DNA. Universal primers U789f (5’-TAGATACCCSSGTAGTCC-3’) and U1068r (5’-CTGACGRCRGCCATGC-3’), targeting both bacteria and archaea, were adapted for pyrosequencing by the addition of sequencing adapters and multiplex identifier (MID) sequences. To minimise PCR bias three individual reactions were performed per template and equimolar amounts of PCR products from each of the three reactions were pooled for pyrosequencing. PCR products were purified using Qiagen PCR Purification Kit (Qiagen Ltd., UK) as per the manufacturer’s instructions. Barcoded samples were pooled and sequenced on a GS FLX Titanium platform. SRS502745 BD188 BD188_LD AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX377940 SNCh_Microbiota SRP033019 PCR amplicon libraries of the V5-V6 region of 16S rRNA genes were prepared from metagenomic DNA. Universal primers U789f (5’-TAGATACCCSSGTAGTCC-3’) and U1068r (5’-CTGACGRCRGCCATGC-3’), targeting both bacteria and archaea, were adapted for pyrosequencing by the addition of sequencing adapters and multiplex identifier (MID) sequences. To minimise PCR bias three individual reactions were performed per template and equimolar amounts of PCR products from each of the three reactions were pooled for pyrosequencing. PCR products were purified using Qiagen PCR Purification Kit (Qiagen Ltd., UK) as per the manufacturer’s instructions. Barcoded samples were pooled and sequenced on a GS FLX Titanium platform. SRS502746 BD243a BD243a_SNCh AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX377959 SNCo_Microbiota SRP033019 PCR amplicon libraries of the V5-V6 region of 16S rRNA genes were prepared from metagenomic DNA. Universal primers U789f (5’-TAGATACCCSSGTAGTCC-3’) and U1068r (5’-CTGACGRCRGCCATGC-3’), targeting both bacteria and archaea, were adapted for pyrosequencing by the addition of sequencing adapters and multiplex identifier (MID) sequences. To minimise PCR bias three individual reactions were performed per template and equimolar amounts of PCR products from each of the three reactions were pooled for pyrosequencing. PCR products were purified using Qiagen PCR Purification Kit (Qiagen Ltd., UK) as per the manufacturer’s instructions. Barcoded samples were pooled and sequenced on a GS FLX Titanium platform. SRS502765 BD243b BD243b_SNCo AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX377967 SW1_Microbiota SRP033019 PRJNA227795 PCR amplicon libraries of the V5-V6 region of 16S rRNA genes were prepared from metagenomic DNA. Universal primers U789f (5’-TAGATACCCSSGTAGTCC-3’) and U1068r (5’-CTGACGRCRGCCATGC-3’), targeting both bacteria and archaea, were adapted for pyrosequencing by the addition of sequencing adapters and multiplex identifier (MID) sequences. To minimise PCR bias three individual reactions were performed per template and equimolar amounts of PCR products from each of the three reactions were pooled for pyrosequencing. PCR products were purified using Qiagen PCR Purification Kit (Qiagen Ltd., UK) as per the manufacturer’s instructions. Barcoded samples were pooled and sequenced on a GS FLX Titanium platform. SRS502773 SAMN02402460 BD76_SW1 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX377968 SW2_Microbiota SRP033019 PRJNA227795 PCR amplicon libraries of the V5-V6 region of 16S rRNA genes were prepared from metagenomic DNA. Universal primers U789f (5’-TAGATACCCSSGTAGTCC-3’) and U1068r (5’-CTGACGRCRGCCATGC-3’), targeting both bacteria and archaea, were adapted for pyrosequencing by the addition of sequencing adapters and multiplex identifier (MID) sequences. To minimise PCR bias three individual reactions were performed per template and equimolar amounts of PCR products from each of the three reactions were pooled for pyrosequencing. PCR products were purified using Qiagen PCR Purification Kit (Qiagen Ltd., UK) as per the manufacturer’s instructions. Barcoded samples were pooled and sequenced on a GS FLX Titanium platform. SRS502774 SAMN02402461 BD116_SW2 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium SRX377969 SW3_Microbiota SRP033019 PCR amplicon libraries of the V5-V6 region of 16S rRNA genes were prepared from metagenomic DNA. Universal primers U789f (5’-TAGATACCCSSGTAGTCC-3’) and U1068r (5’-CTGACGRCRGCCATGC-3’), targeting both bacteria and archaea, were adapted for pyrosequencing by the addition of sequencing adapters and multiplex identifier (MID) sequences. To minimise PCR bias three individual reactions were performed per template and equimolar amounts of PCR products from each of the three reactions were pooled for pyrosequencing. PCR products were purified using Qiagen PCR Purification Kit (Qiagen Ltd., UK) as per the manufacturer’s instructions. Barcoded samples were pooled and sequenced on a GS FLX Titanium platform. SRS502775 BD250 BD250_SW3 AMPLICON METAGENOMIC PCR 0 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX Titanium