SRX378873 P.notoginseng transcriptome root SRP033112 PRJNA228978 Total RNA extracted from the root of notoginseng treats with DNase I, and magnetic beads with Oligo (dT) are used to isolate mRNA. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification to create the final cDNA library template. SRS503442 SAMN02404671 P.notoginseng root RNA-Seq TRANSCRIPTOMIC cDNA 180 0 Application Read Forward 1 1 Application Read Reverse 91 Illumina HiSeq 2000 SRX378878 P.notoginseng transcriptome flower SRP033112 PRJNA228978 Total RNA extracted from the flower of notoginseng treats with DNase I, and magnetic beads with Oligo (dT) are used to isolate mRNA. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification to create the final cDNA library template. SRS503442 SAMN02404671 P.notoginseng flower RNA-Seq TRANSCRIPTOMIC cDNA 180 0 Application Read Forward 1 1 Application Read Reverse 91 Illumina HiSeq 2000 SRX378880 P.notoginseng transcriptome leaf SRP033112 PRJNA228978 Total RNA extracted from the leaf of notoginseng treats with DNase I, and magnetic beads with Oligo (dT) are used to isolate mRNA. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification to create the final cDNA library template. SRS503442 SAMN02404671 P.notoginseng leaf RNA-Seq TRANSCRIPTOMIC cDNA 180 0 Application Read Forward 1 1 Application Read Reverse 91 Illumina HiSeq 2000