SRX9370756 GSM4867297 SRP288754 SRS7592445 GSM4867297 RNA-Seq TRANSCRIPTOMIC cDNA Single cells were suspended in calcium- and magnesium-free PBS containing 0.04% weight/volume BSA. Cells were added to each channel. After generation of GEMs, reverse transcription reactions were engaged barcoded full-length cDNA followed by the disruption of emulsions using the recovery agent and cDNA clean up with DynaBeads Myone Silane Beads (Thermo Fisher Scientific). cDNA was then amplified by PCR with appropriate cycles which depend on the recovery cells. Subsequently, the amplified cDNA was fragmented, end-repaired, A-tailed, and index adaptor ligated and library amplification. Then these libraries were sequenced on the Illumina sequencing platform and 150 bp paired-end reads were generated. RNA libraries were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol. Illumina NovaSeq 6000 gds 304867297 GSM4867297 GEO Accession GSM4867297 SRX9370757 GSM4867299 SRP288754 SRS7592446 GSM4867299 RNA-Seq TRANSCRIPTOMIC cDNA Single cells were suspended in calcium- and magnesium-free PBS containing 0.04% weight/volume BSA. Cells were added to each channel. After generation of GEMs, reverse transcription reactions were engaged barcoded full-length cDNA followed by the disruption of emulsions using the recovery agent and cDNA clean up with DynaBeads Myone Silane Beads (Thermo Fisher Scientific). cDNA was then amplified by PCR with appropriate cycles which depend on the recovery cells. Subsequently, the amplified cDNA was fragmented, end-repaired, A-tailed, and index adaptor ligated and library amplification. Then these libraries were sequenced on the Illumina sequencing platform and 150 bp paired-end reads were generated. RNA libraries were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol. Illumina NovaSeq 6000 gds 304867299 GSM4867299 GEO Accession GSM4867299