SRP298793 PRJNA686430 Characterisation of hepatic transcriptomic profiles in low-fat and high fat diet fed C57BL/6J male mice with or without (-)-epicatechin or 3',4'-dihydroxyphenyl-y-valerolactone supplementation We report a RNA-sequencing experiment which aimed to identify how a parent flavan-3-ol ((-)-epicatechin) and their colonic derived hydroxyphenyl-y-valerolactone ring fission metabolites can protect against metabolic associated fatty liver disease (MAFLD) and hepatic insulin resistance. C56BL/6J male mice were fed diets of low-fat (LF) (10 percent kcal of fat) or high-fat (HF) (60 percent kcal of fat) for 15-weeks and used to study the effects of 20 mg/kg body weight supplementation of epicatechin (EC) or 3',4'-dihydroxyphenyl-y-valerolactone (GVL) in livers. This was aimed to specifically investigate the hepatic transcriptional events that support the actions of EC on protecting against insulin resistance effects, and to identify whether GVL exerts a similar response in mice fed HF or LF diets. Total RNA was extracted from the mice livers and was sequenced using Illumina NovaSeq 6000 for 7 biological replicates of each dietary treatment groups: LF (control), LF-EC, LF-GVL, HF (control), HF-EC and HF-GVL.