<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT alias="GSM4976172" accession="SRX9687521">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX9687521</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM4976172</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM4976172: Smutans_2hr_Media_1; Streptococcus mutans; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP298036" refname="GSE163258">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP298036</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS7885096">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS7885096</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM4976172</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Pellets were thawed on ice and resuspended in RNAase free water, 50 mL of 0.1 mm silica spheres (MP Lysing Matrix B) were added, and samples were bead-beaten for 90 seconds in three intervals at 4°C. Total RNA was then extracted using the MasterPure Complete RNA Purification kit (Lucigen). Genomic DNA was removed using the Turbo DNA-free kit (Ambion). Total RNA was measured with an Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were prepared using the Ribo-Zero Magnetic Kit (Bacteria; Epicentre) and the KAPA RNA HyperPrep kit (Kapa Biosystems). Library sequencing was performed using the Illumina HiSeq 2000 platform with a single-end protocol and read lengths of 40 nucleotides. Samples were stored at -20°C. Total RNA (depleted of rRNA) was converted to cDNA and sequenced with Illumina HiSeq 2000 with a single-end protocol and read lengths of 40 nucleotides</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>304976172</ID>
          <LABEL>GSM4976172</LABEL>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM4976172</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM4976173" accession="SRX9687522">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX9687522</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM4976173</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM4976173: Smutans_2hr_Media_2; Streptococcus mutans; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP298036" refname="GSE163258">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP298036</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS7885097">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS7885097</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM4976173</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Pellets were thawed on ice and resuspended in RNAase free water, 50 mL of 0.1 mm silica spheres (MP Lysing Matrix B) were added, and samples were bead-beaten for 90 seconds in three intervals at 4°C. Total RNA was then extracted using the MasterPure Complete RNA Purification kit (Lucigen). Genomic DNA was removed using the Turbo DNA-free kit (Ambion). Total RNA was measured with an Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were prepared using the Ribo-Zero Magnetic Kit (Bacteria; Epicentre) and the KAPA RNA HyperPrep kit (Kapa Biosystems). Library sequencing was performed using the Illumina HiSeq 2000 platform with a single-end protocol and read lengths of 40 nucleotides. Samples were stored at -20°C. Total RNA (depleted of rRNA) was converted to cDNA and sequenced with Illumina HiSeq 2000 with a single-end protocol and read lengths of 40 nucleotides</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>304976173</ID>
          <LABEL>GSM4976173</LABEL>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM4976173</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM4976174" accession="SRX9687523">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX9687523</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM4976174</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM4976174: Smutans_2hr_MUC5Bmucins_1; Streptococcus mutans; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP298036" refname="GSE163258">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP298036</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS7885098">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS7885098</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM4976174</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Pellets were thawed on ice and resuspended in RNAase free water, 50 mL of 0.1 mm silica spheres (MP Lysing Matrix B) were added, and samples were bead-beaten for 90 seconds in three intervals at 4°C. Total RNA was then extracted using the MasterPure Complete RNA Purification kit (Lucigen). Genomic DNA was removed using the Turbo DNA-free kit (Ambion). Total RNA was measured with an Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were prepared using the Ribo-Zero Magnetic Kit (Bacteria; Epicentre) and the KAPA RNA HyperPrep kit (Kapa Biosystems). Library sequencing was performed using the Illumina HiSeq 2000 platform with a single-end protocol and read lengths of 40 nucleotides. Samples were stored at -20°C. Total RNA (depleted of rRNA) was converted to cDNA and sequenced with Illumina HiSeq 2000 with a single-end protocol and read lengths of 40 nucleotides</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>304976174</ID>
          <LABEL>GSM4976174</LABEL>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM4976174</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM4976175" accession="SRX9687524">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX9687524</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM4976175</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM4976175: Smutans_2hr_MUC5Bmucins_2; Streptococcus mutans; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP298036" refname="GSE163258">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP298036</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS7885099">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS7885099</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM4976175</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Pellets were thawed on ice and resuspended in RNAase free water, 50 mL of 0.1 mm silica spheres (MP Lysing Matrix B) were added, and samples were bead-beaten for 90 seconds in three intervals at 4°C. Total RNA was then extracted using the MasterPure Complete RNA Purification kit (Lucigen). Genomic DNA was removed using the Turbo DNA-free kit (Ambion). Total RNA was measured with an Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were prepared using the Ribo-Zero Magnetic Kit (Bacteria; Epicentre) and the KAPA RNA HyperPrep kit (Kapa Biosystems). Library sequencing was performed using the Illumina HiSeq 2000 platform with a single-end protocol and read lengths of 40 nucleotides. Samples were stored at -20°C. Total RNA (depleted of rRNA) was converted to cDNA and sequenced with Illumina HiSeq 2000 with a single-end protocol and read lengths of 40 nucleotides</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>304976175</ID>
          <LABEL>GSM4976175</LABEL>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM4976175</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM4976176" accession="SRX9687525">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX9687525</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM4976176</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM4976176: Smutans_2hr_MUC5Bglycans_1; Streptococcus mutans; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP298036" refname="GSE163258">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP298036</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS7885100">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS7885100</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM4976176</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Pellets were thawed on ice and resuspended in RNAase free water, 50 mL of 0.1 mm silica spheres (MP Lysing Matrix B) were added, and samples were bead-beaten for 90 seconds in three intervals at 4°C. Total RNA was then extracted using the MasterPure Complete RNA Purification kit (Lucigen). Genomic DNA was removed using the Turbo DNA-free kit (Ambion). Total RNA was measured with an Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were prepared using the Ribo-Zero Magnetic Kit (Bacteria; Epicentre) and the KAPA RNA HyperPrep kit (Kapa Biosystems). Library sequencing was performed using the Illumina HiSeq 2000 platform with a single-end protocol and read lengths of 40 nucleotides. Samples were stored at -20°C. Total RNA (depleted of rRNA) was converted to cDNA and sequenced with Illumina HiSeq 2000 with a single-end protocol and read lengths of 40 nucleotides</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>304976176</ID>
          <LABEL>GSM4976176</LABEL>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM4976176</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM4976177" accession="SRX9687526">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX9687526</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM4976177</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM4976177: Smutans_2hr_MUC5Bglycans_2; Streptococcus mutans; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP298036" refname="GSE163258">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP298036</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS7885101">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS7885101</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM4976177</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Pellets were thawed on ice and resuspended in RNAase free water, 50 mL of 0.1 mm silica spheres (MP Lysing Matrix B) were added, and samples were bead-beaten for 90 seconds in three intervals at 4°C. Total RNA was then extracted using the MasterPure Complete RNA Purification kit (Lucigen). Genomic DNA was removed using the Turbo DNA-free kit (Ambion). Total RNA was measured with an Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were prepared using the Ribo-Zero Magnetic Kit (Bacteria; Epicentre) and the KAPA RNA HyperPrep kit (Kapa Biosystems). Library sequencing was performed using the Illumina HiSeq 2000 platform with a single-end protocol and read lengths of 40 nucleotides. Samples were stored at -20°C. Total RNA (depleted of rRNA) was converted to cDNA and sequenced with Illumina HiSeq 2000 with a single-end protocol and read lengths of 40 nucleotides</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>304976177</ID>
          <LABEL>GSM4976177</LABEL>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM4976177</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM4976178" accession="SRX9687527">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX9687527</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM4976178</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM4976178: Smutans_2hr_MUC5ACglycans_1; Streptococcus mutans; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP298036" refname="GSE163258">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP298036</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS7885102">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS7885102</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM4976178</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Pellets were thawed on ice and resuspended in RNAase free water, 50 mL of 0.1 mm silica spheres (MP Lysing Matrix B) were added, and samples were bead-beaten for 90 seconds in three intervals at 4°C. Total RNA was then extracted using the MasterPure Complete RNA Purification kit (Lucigen). Genomic DNA was removed using the Turbo DNA-free kit (Ambion). Total RNA was measured with an Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were prepared using the Ribo-Zero Magnetic Kit (Bacteria; Epicentre) and the KAPA RNA HyperPrep kit (Kapa Biosystems). Library sequencing was performed using the Illumina HiSeq 2000 platform with a single-end protocol and read lengths of 40 nucleotides. Samples were stored at -20°C. Total RNA (depleted of rRNA) was converted to cDNA and sequenced with Illumina HiSeq 2000 with a single-end protocol and read lengths of 40 nucleotides</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>304976178</ID>
          <LABEL>GSM4976178</LABEL>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM4976178</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM4976179" accession="SRX9687528">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX9687528</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM4976179</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM4976179: Smutans_2hr_MUC5ACglycans_2; Streptococcus mutans; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP298036" refname="GSE163258">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP298036</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS7885103">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS7885103</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM4976179</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Pellets were thawed on ice and resuspended in RNAase free water, 50 mL of 0.1 mm silica spheres (MP Lysing Matrix B) were added, and samples were bead-beaten for 90 seconds in three intervals at 4°C. Total RNA was then extracted using the MasterPure Complete RNA Purification kit (Lucigen). Genomic DNA was removed using the Turbo DNA-free kit (Ambion). Total RNA was measured with an Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were prepared using the Ribo-Zero Magnetic Kit (Bacteria; Epicentre) and the KAPA RNA HyperPrep kit (Kapa Biosystems). Library sequencing was performed using the Illumina HiSeq 2000 platform with a single-end protocol and read lengths of 40 nucleotides. Samples were stored at -20°C. Total RNA (depleted of rRNA) was converted to cDNA and sequenced with Illumina HiSeq 2000 with a single-end protocol and read lengths of 40 nucleotides</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>304976179</ID>
          <LABEL>GSM4976179</LABEL>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
    <EXPERIMENT_ATTRIBUTES>
      <EXPERIMENT_ATTRIBUTE>
        <TAG>GEO Accession</TAG>
        <VALUE>GSM4976179</VALUE>
      </EXPERIMENT_ATTRIBUTE>
    </EXPERIMENT_ATTRIBUTES>
  </EXPERIMENT>
</EXPERIMENT_SET>
