SRX9743641 F7T60C F7 60 replicate C SRP299501 PRJNA688285 A total of 288 ng of DNA was used for the production of paired-end libraries and for sequencing the V4V5 hypervariable regions of the bacterial 16S rRNA gene by using as primers 515F forward primer (5-GTGCCAGCMGCCG CGGTAA-3) and 907R reverse primer (5-CCGTCAATTCCTTTGAGTTT-3). SRS7931510 F7T60C F7T60C AMPLICON METAGENOMIC PCR Illumina MiSeq