SRX9744786 FF1T90C F1 90 replicate C ITS SRP299543 PRJNA688315 A total of 288 ng of DNA was used for the production of paired-end libraries and for sequencing 18S rDNA Internal Spacer region using the ITS1-5F forward primer (5-175 GGAAGTAAAAGTCGTAACAAGG-3) and ITS2-2043R reverse primer (5-176 GCTGCGTTCTTCATCGATGC-3). SRS7932443 FF1T90C FF1T90C AMPLICON METAGENOMIC PCR Illumina MiSeq