SRP301282 PRJNA690973 Role of microbial bile tolerance trait in colonization resistance against Clostridium difficile infection Currently, US-FDA has only approved antibiotics against C. difficile infection which is associated with relapse of the disease. Previous studies have emphasized the importance of colonization resistance by the gut microbiota against C. difficile. One of the ways to restore the colonization resistance is using defined microbial consortia. However, such consortia previously have reported bacterial colonization issues. One of the host factors affecting the colonization of the bacteria in the gut is bile. However, it has been fairly overlooked during the designing of the consortia against C. difficile. Here, we phenotypically screened the cultured human gut library from our previous study in vitro to categorize the high bile tolerant and low bile tolerant fraction of the gut microbiota and test the subsequent bacterial consortia against CDI in vitro.