SRS8379967 SAMN18139862 7227 Drosophila melanogaster bioproject 706669 strain w1118_(wt) dev_stage adult sex male tissue sperm biomaterial_provider Anita st lab, BKV, Linkping University, Sweden breeding_method Flies were maintained on standard-diet at 25C. 0-4 days old flies were manually sorted to 15 males and 15 females per vial (or 45+45 in bottles) and allowed to lay eggs for 4 days. Flies were crossed on Thursday, flipped on Monday and virgins collected the following Monday. On Thursday flies were crossed again establishing a 2-week generation cycle. Genetic background was equalized between mutant and WT strains by crossing the mutant lines over double balancer lines where chromosomes were followed with a visible marker. This way crosses were generated in which all chromosomes were replaced with WT chromosomes, except the one carrying the mutation and the respective balancer. Flies from this cross were then crossed with WT, where offspring without the balancer chromosome was used for the hetrozygot mutant experiment. growth_protocol Standard diet: Agar 12 g/l, yeast 18 g/l, soy flour 10 g/l, yellow cornmeal 80 g/l, propionic acid 6,25 ml/l, molasses 22 g/l, malt extract 80 g/l, Nipagin 24 g/l, 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BioSampleModel Model organism or animal SRS8379968 SAMN18139866 7227 Drosophila melanogaster bioproject 706669 strain w1118_(wt) dev_stage adult sex male tissue Testicle_part_2 biomaterial_provider Anita st lab, BKV, Linkping University, Sweden breeding_method Flies were maintained on standard-diet at 25C. 0-4 days old flies were manually sorted to 15 males and 15 females per vial (or 45+45 in bottles) and allowed to lay eggs for 4 days. Flies were crossed on Thursday, flipped on Monday and virgins collected the following Monday. On Thursday flies were crossed again establishing a 2-week generation cycle. Genetic background was equalized between mutant and WT strains by crossing the mutant lines over double balancer lines where chromosomes were followed with a visible marker. This way crosses were generated in which all chromosomes were replaced with WT chromosomes, except the one carrying the mutation and the respective balancer. Flies from this cross were then crossed with WT, where offspring without the balancer chromosome was used for the hetrozygot mutant experiment. growth_protocol Standard diet: Agar 12 g/l, yeast 18 g/l, soy flour 10 g/l, yellow cornmeal 80 g/l, propionic acid 6,25 ml/l, molasses 22 g/l, malt extract 80 g/l, Nipagin 24 g/l, 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BioSampleModel Model organism or animal SRS8379969 SAMN18139867 7227 Drosophila melanogaster bioproject 706669 strain w1118_(wt) dev_stage adult sex male tissue Testicle_part_3 biomaterial_provider Anita st lab, BKV, Linkping University, Sweden breeding_method Flies were maintained on standard-diet at 25C. 0-4 days old flies were manually sorted to 15 males and 15 females per vial (or 45+45 in bottles) and allowed to lay eggs for 4 days. Flies were crossed on Thursday, flipped on Monday and virgins collected the following Monday. On Thursday flies were crossed again establishing a 2-week generation cycle. Genetic background was equalized between mutant and WT strains by crossing the mutant lines over double balancer lines where chromosomes were followed with a visible marker. This way crosses were generated in which all chromosomes were replaced with WT chromosomes, except the one carrying the mutation and the respective balancer. Flies from this cross were then crossed with WT, where offspring without the balancer chromosome was used for the hetrozygot mutant experiment. growth_protocol Standard diet: Agar 12 g/l, yeast 18 g/l, soy flour 10 g/l, yellow cornmeal 80 g/l, propionic acid 6,25 ml/l, molasses 22 g/l, malt extract 80 g/l, Nipagin 24 g/l, 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BioSampleModel Model organism or animal SRS8379970 SAMN18139865 7227 Drosophila melanogaster bioproject 706669 strain w1118_(wt) dev_stage adult sex male tissue Testicle_part_1 biomaterial_provider Anita st lab, BKV, Linkping University, Sweden breeding_method Flies were maintained on standard-diet at 25C. 0-4 days old flies were manually sorted to 15 males and 15 females per vial (or 45+45 in bottles) and allowed to lay eggs for 4 days. Flies were crossed on Thursday, flipped on Monday and virgins collected the following Monday. On Thursday flies were crossed again establishing a 2-week generation cycle. Genetic background was equalized between mutant and WT strains by crossing the mutant lines over double balancer lines where chromosomes were followed with a visible marker. This way crosses were generated in which all chromosomes were replaced with WT chromosomes, except the one carrying the mutation and the respective balancer. Flies from this cross were then crossed with WT, where offspring without the balancer chromosome was used for the hetrozygot mutant experiment. growth_protocol Standard diet: Agar 12 g/l, yeast 18 g/l, soy flour 10 g/l, yellow cornmeal 80 g/l, propionic acid 6,25 ml/l, molasses 22 g/l, malt extract 80 g/l, Nipagin 24 g/l, 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BioSampleModel Model organism or animal SRS8379971 SAMN18139863 7227 Drosophila melanogaster bioproject 706669 strain hetero_wt/piwi1 dev_stage adult sex male tissue sperm biomaterial_provider Bloomington drosophila stock center (#43637), Indiana University, USA breeding_method Flies were maintained on standard-diet at 25C. 0-4 days old flies were manually sorted to 15 males and 15 females per vial (or 45+45 in bottles) and allowed to lay eggs for 4 days. Flies were crossed on Thursday, flipped on Monday and virgins collected the following Monday. On Thursday flies were crossed again establishing a 2-week generation cycle. Genetic background was equalized between mutant and WT strains by crossing the mutant lines over double balancer lines where chromosomes were followed with a visible marker. This way crosses were generated in which all chromosomes were replaced with WT chromosomes, except the one carrying the mutation and the respective balancer. Flies from this cross were then crossed with WT, where offspring without the balancer chromosome was used for the hetrozygot mutant experiment. growth_protocol Standard diet: Agar 12 g/l, yeast 18 g/l, soy flour 10 g/l, yellow cornmeal 80 g/l, propionic acid 6,25 ml/l, molasses 22 g/l, malt extract 80 g/l, Nipagin 24 g/l, 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BioSampleModel Model organism or animal SRS8379972 SAMN18139864 7227 Drosophila melanogaster bioproject 706669 strain hetero_wt/aubQc42 dev_stage adult sex male tissue sperm biomaterial_provider Bloomington drosophila stock center (#4968), Indiana University, USA breeding_method Flies were maintained on standard-diet at 25C. 0-4 days old flies were manually sorted to 15 males and 15 females per vial (or 45+45 in bottles) and allowed to lay eggs for 4 days. Flies were crossed on Thursday, flipped on Monday and virgins collected the following Monday. On Thursday flies were crossed again establishing a 2-week generation cycle. Genetic background was equalized between mutant and WT strains by crossing the mutant lines over double balancer lines where chromosomes were followed with a visible marker. This way crosses were generated in which all chromosomes were replaced with WT chromosomes, except the one carrying the mutation and the respective balancer. Flies from this cross were then crossed with WT, where offspring without the balancer chromosome was used for the hetrozygot mutant experiment. growth_protocol Standard diet: Agar 12 g/l, yeast 18 g/l, soy flour 10 g/l, yellow cornmeal 80 g/l, propionic acid 6,25 ml/l, molasses 22 g/l, malt extract 80 g/l, Nipagin 24 g/l, 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BioSampleModel Model organism or animal