SRX10313918 GSM5159844 SRP310249 SRS8434364 GSM5159844 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159844 GSM5159844 GEO Accession GSM5159844 SRX10313919 GSM5159845 SRP310249 SRS8434365 GSM5159845 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159845 GSM5159845 GEO Accession GSM5159845 SRX10313920 GSM5159846 SRP310249 SRS8434366 GSM5159846 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159846 GSM5159846 GEO Accession GSM5159846 SRX10313921 GSM5159847 SRP310249 SRS8434367 GSM5159847 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159847 GSM5159847 GEO Accession GSM5159847 SRX10313922 GSM5159848 SRP310249 SRS8434368 GSM5159848 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159848 GSM5159848 GEO Accession GSM5159848 SRX10313923 GSM5159849 SRP310249 SRS8434369 GSM5159849 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159849 GSM5159849 GEO Accession GSM5159849 SRX10313924 GSM5159850 SRP310249 SRS8434370 GSM5159850 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159850 GSM5159850 GEO Accession GSM5159850 SRX10313925 GSM5159851 SRP310249 SRS8434371 GSM5159851 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159851 GSM5159851 GEO Accession GSM5159851 SRX10313926 GSM5159852 SRP310249 SRS8434372 GSM5159852 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159852 GSM5159852 GEO Accession GSM5159852 SRX10313927 GSM5159853 SRP310249 SRS8434373 GSM5159853 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159853 GSM5159853 GEO Accession GSM5159853 SRX10313928 GSM5159854 SRP310249 SRS8434374 GSM5159854 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159854 GSM5159854 GEO Accession GSM5159854 SRX10313929 GSM5159855 SRP310249 SRS8434375 GSM5159855 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159855 GSM5159855 GEO Accession GSM5159855 SRX10313930 GSM5159856 SRP310249 SRS8434377 GSM5159856 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159856 GSM5159856 GEO Accession GSM5159856 SRX10313931 GSM5159857 SRP310249 SRS8434376 GSM5159857 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159857 GSM5159857 GEO Accession GSM5159857 SRX10313932 GSM5159858 SRP310249 SRS8434378 GSM5159858 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159858 GSM5159858 GEO Accession GSM5159858 SRX10313933 GSM5159859 SRP310249 SRS8434379 GSM5159859 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159859 GSM5159859 GEO Accession GSM5159859 SRX10313934 GSM5159860 SRP310249 SRS8434380 GSM5159860 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159860 GSM5159860 GEO Accession GSM5159860 SRX10313935 GSM5159861 SRP310249 SRS8434381 GSM5159861 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159861 GSM5159861 GEO Accession GSM5159861 SRX10313936 GSM5159862 SRP310249 SRS8434383 GSM5159862 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159862 GSM5159862 GEO Accession GSM5159862 SRX10313937 GSM5159863 SRP310249 SRS8434382 GSM5159863 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159863 GSM5159863 GEO Accession GSM5159863 SRX10313938 GSM5159864 SRP310249 SRS8434384 GSM5159864 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159864 GSM5159864 GEO Accession GSM5159864 SRX10313939 GSM5159865 SRP310249 SRS8434385 GSM5159865 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159865 GSM5159865 GEO Accession GSM5159865 SRX10313940 GSM5159866 SRP310249 SRS8434388 GSM5159866 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159866 GSM5159866 GEO Accession GSM5159866 SRX10313941 GSM5159867 SRP310249 SRS8434386 GSM5159867 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159867 GSM5159867 GEO Accession GSM5159867 SRX10313942 GSM5159868 SRP310249 SRS8434387 GSM5159868 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159868 GSM5159868 GEO Accession GSM5159868 SRX10313943 GSM5159869 SRP310249 SRS8434389 GSM5159869 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159869 GSM5159869 GEO Accession GSM5159869 SRX10313944 GSM5159870 SRP310249 SRS8434391 GSM5159870 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159870 GSM5159870 GEO Accession GSM5159870 SRX10313945 GSM5159871 SRP310249 SRS8434390 GSM5159871 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159871 GSM5159871 GEO Accession GSM5159871 SRX10313946 GSM5159872 SRP310249 SRS8434392 GSM5159872 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159872 GSM5159872 GEO Accession GSM5159872 SRX10313947 GSM5159873 SRP310249 SRS8434394 GSM5159873 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159873 GSM5159873 GEO Accession GSM5159873 SRX10313948 GSM5159874 SRP310249 SRS8434393 GSM5159874 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159874 GSM5159874 GEO Accession GSM5159874 SRX10313949 GSM5159875 SRP310249 SRS8434395 GSM5159875 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested by two different protocols. For normal RNA sequencing, cells were resuspended in Trizol reagent, followed by chloroform extraction and isopropanol precipitation of the RNA. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from untreated polysome fractions, each fraction went through 1 phenol:chlorofom isoamyl alcohol extraction and 2 chloroform extractions. RNA was isopropanol precipitated, fractions were combined, and genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Polysome-associated RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. To isolate RNA from stressed polysome fractions, each fraction was ethanol precipitated, resuspended and fractions were combined, and then samples were TRIzol extracted. Genomic DNA was depleted using Turbo DNase, followed by cleanup with RNAclean beads. Total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 and Kiefer et al. Journal of the American Chemical Society 2018. RNA sequencing libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). Illumina NovaSeq 6000 gds 305159875 GSM5159875 GEO Accession GSM5159875