SRX10324191 WT_0_1 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444673 WT_0_1 WT_0_1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324192 WT_0_2 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444674 WT_0_2 WT_0_2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324193 KO_60_2 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444679 KO_60_2 KO_60_2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324194 KO_60_3 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444675 KO_60_3 KO_60_3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324195 WT_0_3 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444676 WT_0_3 WT_0_3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324196 WT_60_1 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444678 WT_60_1 WT_60_1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324197 WT_60_2 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444677 WT_60_2 WT_60_2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324198 WT_60_3 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444681 WT_60_3 WT_60_3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324199 KO_0_1 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444680 KO_0_1 KO_0_1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324200 KO_0_2 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444682 KO_0_2 KO_0_2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324201 KO_0_3 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444683 KO_0_3 KO_0_3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000 SRX10324202 KO_60_1 SRP310313 bp0 Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 10 ug of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp). And then we performed the paired-end sequencing on an Illumina Novaseq 6000 at the (lc-bio, China) following the vendor's recommended protocol. SRS8444684 KO_60_1 KO_60_1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 4000