SRX10331417 GSM5170162 SRP310405 SRS8451152 GSM5170162 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170162 GSM5170162 GEO Accession GSM5170162 SRX10331418 GSM5170163 SRP310405 SRS8451150 GSM5170163 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170163 GSM5170163 GEO Accession GSM5170163 SRX10331419 GSM5170164 SRP310405 SRS8451153 GSM5170164 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170164 GSM5170164 GEO Accession GSM5170164 SRX10331420 GSM5170165 SRP310405 SRS8451151 GSM5170165 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170165 GSM5170165 GEO Accession GSM5170165 SRX10331421 GSM5170166 SRP310405 SRS8451154 GSM5170166 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170166 GSM5170166 GEO Accession GSM5170166 SRX10331422 GSM5170167 SRP310405 SRS8451155 GSM5170167 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170167 GSM5170167 GEO Accession GSM5170167 SRX10331423 GSM5170168 SRP310405 SRS8451156 GSM5170168 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170168 GSM5170168 GEO Accession GSM5170168 SRX10331424 GSM5170169 SRP310405 SRS8451157 GSM5170169 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170169 GSM5170169 GEO Accession GSM5170169 SRX10331425 GSM5170170 SRP310405 SRS8451158 GSM5170170 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170170 GSM5170170 GEO Accession GSM5170170 SRX10331426 GSM5170171 SRP310405 SRS8451160 GSM5170171 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170171 GSM5170171 GEO Accession GSM5170171 SRX10331427 GSM5170172 SRP310405 SRS8451159 GSM5170172 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170172 GSM5170172 GEO Accession GSM5170172 SRX10331428 GSM5170173 SRP310405 SRS8451161 GSM5170173 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170173 GSM5170173 GEO Accession GSM5170173 SRX10331429 GSM5170174 SRP310405 SRS8451162 GSM5170174 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170174 GSM5170174 GEO Accession GSM5170174 SRX10331430 GSM5170175 SRP310405 SRS8451163 GSM5170175 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170175 GSM5170175 GEO Accession GSM5170175 SRX10331431 GSM5170176 SRP310405 SRS8451164 GSM5170176 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170176 GSM5170176 GEO Accession GSM5170176 SRX10331432 GSM5170177 SRP310405 SRS8451165 GSM5170177 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170177 GSM5170177 GEO Accession GSM5170177 SRX10331433 GSM5170178 SRP310405 SRS8451166 GSM5170178 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170178 GSM5170178 GEO Accession GSM5170178 SRX10331434 GSM5170179 SRP310405 SRS8451167 GSM5170179 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170179 GSM5170179 GEO Accession GSM5170179 SRX10331435 GSM5170180 SRP310405 SRS8451168 GSM5170180 RNA-Seq TRANSCRIPTOMIC cDNA Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche). Illumina HiSeq 2500 gds 305170180 GSM5170180 GEO Accession GSM5170180