SRX10331924 GSM5170563 SRP310417 SRS8451642 GSM5170563 RNA-Seq TRANSCRIPTOMIC cDNA Single cell suspension from mouse colon was obtained by enzymatic digestion following the Miltenyi Biotec Lamina Propria Dissociation Kit (130-097-410). Cells then were stained with both flow cytometry and CITEseq (HTO and ADTs) antibodies prior sorting to enrich for CD45+ population (80% CD45+B220-, 10% CD45+B220+, 10% CD45-) for each treatment group. Sorted cells then were loaded in the Chromium 10x genomics controller following manufacturer V2 protocol. A single cell library from each experimental group was prepared following the 10x genomics V2 protocol. Material was QC and quantified by TapeStation D5000 (Agilent) prior to sequencing using a NextSeq 500 (Illumina). The final library was a combination of each sublibrary in a proportion 21.5:1.25:2.5 (Transcriptome: HTO: ADT) from each one. NextSeq 550 gds 305170563 GSM5170563 GEO Accession GSM5170563 SRX10331925 GSM5170564 SRP310417 SRS8451640 GSM5170564 RNA-Seq TRANSCRIPTOMIC cDNA Single cell suspension from mouse colon was obtained by enzymatic digestion following the Miltenyi Biotec Lamina Propria Dissociation Kit (130-097-410). Cells then were stained with both flow cytometry and CITEseq (HTO and ADTs) antibodies prior sorting to enrich for CD45+ population (80% CD45+B220-, 10% CD45+B220+, 10% CD45-) for each treatment group. Sorted cells then were loaded in the Chromium 10x genomics controller following manufacturer V2 protocol. A single cell library from each experimental group was prepared following the 10x genomics V2 protocol. Material was QC and quantified by TapeStation D5000 (Agilent) prior to sequencing using a NextSeq 500 (Illumina). The final library was a combination of each sublibrary in a proportion 21.5:1.25:2.5 (Transcriptome: HTO: ADT) from each one. NextSeq 550 gds 305170564 GSM5170564 GEO Accession GSM5170564 SRX10331926 GSM5170565 SRP310417 SRS8451639 GSM5170565 RNA-Seq TRANSCRIPTOMIC cDNA Single cell suspension from mouse colon was obtained by enzymatic digestion following the Miltenyi Biotec Lamina Propria Dissociation Kit (130-097-410). Cells then were stained with both flow cytometry and CITEseq (HTO and ADTs) antibodies prior sorting to enrich for CD45+ population (80% CD45+B220-, 10% CD45+B220+, 10% CD45-) for each treatment group. Sorted cells then were loaded in the Chromium 10x genomics controller following manufacturer V2 protocol. A single cell library from each experimental group was prepared following the 10x genomics V2 protocol. Material was QC and quantified by TapeStation D5000 (Agilent) prior to sequencing using a NextSeq 500 (Illumina). The final library was a combination of each sublibrary in a proportion 21.5:1.25:2.5 (Transcriptome: HTO: ADT) from each one. NextSeq 550 gds 305170565 GSM5170565 GEO Accession GSM5170565 SRX10331927 GSM5170566 SRP310417 SRS8451643 GSM5170566 RNA-Seq TRANSCRIPTOMIC cDNA Single cell suspension from mouse colon was obtained by enzymatic digestion following the Miltenyi Biotec Lamina Propria Dissociation Kit (130-097-410). Cells then were stained with both flow cytometry and CITEseq (HTO and ADTs) antibodies prior sorting to enrich for CD45+ population (80% CD45+B220-, 10% CD45+B220+, 10% CD45-) for each treatment group. Sorted cells then were loaded in the Chromium 10x genomics controller following manufacturer V2 protocol. A single cell library from each experimental group was prepared following the 10x genomics V2 protocol. Material was QC and quantified by TapeStation D5000 (Agilent) prior to sequencing using a NextSeq 500 (Illumina). The final library was a combination of each sublibrary in a proportion 21.5:1.25:2.5 (Transcriptome: HTO: ADT) from each one. NextSeq 550 gds 305170566 GSM5170566 GEO Accession GSM5170566 SRX10331928 GSM5170567 SRP310417 SRS8451641 GSM5170567 RNA-Seq TRANSCRIPTOMIC cDNA Single cell suspension from mouse colon was obtained by enzymatic digestion following the Miltenyi Biotec Lamina Propria Dissociation Kit (130-097-410). Cells then were stained with both flow cytometry and CITEseq (HTO and ADTs) antibodies prior sorting to enrich for CD45+ population (80% CD45+B220-, 10% CD45+B220+, 10% CD45-) for each treatment group. Sorted cells then were loaded in the Chromium 10x genomics controller following manufacturer V2 protocol. A single cell library from each experimental group was prepared following the 10x genomics V2 protocol. Material was QC and quantified by TapeStation D5000 (Agilent) prior to sequencing using a NextSeq 500 (Illumina). The final library was a combination of each sublibrary in a proportion 21.5:1.25:2.5 (Transcriptome: HTO: ADT) from each one. NextSeq 550 gds 305170567 GSM5170567 GEO Accession GSM5170567