SRP310484 PRJNA714193 Zebrafish VTG1 knockout Vitellogenins (Vtgs) are essential for female reproduction in oviparous animals, yet the exact roles and mechanisms remain unknown. In the present study, we knocked out vtg1, which is the most abundant Vtg in zebrafish, via the CRISPR/Cas 9 technology. In female broodstock, the Vtg1-deficiency inhibited docosahexaenoic acid-enriched phosphatidylcholine (DHA-PC) transportation from liver to ovary, thus resulted in lower DHA-PC contents in ovary and larval offspring.