SRX10386347 GSM5184289 SRP311353 SRS8509478 GSM5184289 RNA-Seq TRANSCRIPTOMIC CAGE Adult S. purpuratus were obtained from Pat Leahy (Caltech). Gametes were collected by intracoelomic injection of 0.5 M KCl. Fertilization and embryo culture were carried out according to Adams et al. (2019). Embryos were cultured at 15° C in 4-liter plastic beakers fitted with battery powered stirrers. Embryos were harvested at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr post- fertilization (hpf). The 9 samples were derived from two separate embryo cultures. For the 0 hpf time point, embryos were collected immediately after fertilization. Total RNA (75-100 g/sample) was isolated using the RNeasy Plus Mini kit (Qiagen Cat. No. 74134) and the quality of the preparations was confirmed using an RNA TapeStation (Agilent). RNA integrity numbers (RINs) for all samples were 9.8-10.0. RNA samples were stored at -80°C. CAGE library preparation, sequencing, mapping, and eRNA identification were performed by DNAFORM (Yokohama, Kanagawa, Japan) Illumina HiSeq 2000 gds 305184289 GSM5184289 GEO Accession GSM5184289 SRX10386348 GSM5184290 SRP311353 SRS8509479 GSM5184290 RNA-Seq TRANSCRIPTOMIC CAGE Adult S. purpuratus were obtained from Pat Leahy (Caltech). Gametes were collected by intracoelomic injection of 0.5 M KCl. Fertilization and embryo culture were carried out according to Adams et al. (2019). Embryos were cultured at 15° C in 4-liter plastic beakers fitted with battery powered stirrers. Embryos were harvested at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr post- fertilization (hpf). The 9 samples were derived from two separate embryo cultures. For the 0 hpf time point, embryos were collected immediately after fertilization. Total RNA (75-100 g/sample) was isolated using the RNeasy Plus Mini kit (Qiagen Cat. No. 74134) and the quality of the preparations was confirmed using an RNA TapeStation (Agilent). RNA integrity numbers (RINs) for all samples were 9.8-10.0. RNA samples were stored at -80°C. CAGE library preparation, sequencing, mapping, and eRNA identification were performed by DNAFORM (Yokohama, Kanagawa, Japan) Illumina HiSeq 2000 gds 305184290 GSM5184290 GEO Accession GSM5184290 SRX10386349 GSM5184291 SRP311353 SRS8509480 GSM5184291 RNA-Seq TRANSCRIPTOMIC CAGE Adult S. purpuratus were obtained from Pat Leahy (Caltech). Gametes were collected by intracoelomic injection of 0.5 M KCl. Fertilization and embryo culture were carried out according to Adams et al. (2019). Embryos were cultured at 15° C in 4-liter plastic beakers fitted with battery powered stirrers. Embryos were harvested at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr post- fertilization (hpf). The 9 samples were derived from two separate embryo cultures. For the 0 hpf time point, embryos were collected immediately after fertilization. Total RNA (75-100 g/sample) was isolated using the RNeasy Plus Mini kit (Qiagen Cat. No. 74134) and the quality of the preparations was confirmed using an RNA TapeStation (Agilent). RNA integrity numbers (RINs) for all samples were 9.8-10.0. RNA samples were stored at -80°C. CAGE library preparation, sequencing, mapping, and eRNA identification were performed by DNAFORM (Yokohama, Kanagawa, Japan) Illumina HiSeq 2000 gds 305184291 GSM5184291 GEO Accession GSM5184291 SRX10386350 GSM5184292 SRP311353 SRS8509481 GSM5184292 RNA-Seq TRANSCRIPTOMIC CAGE Adult S. purpuratus were obtained from Pat Leahy (Caltech). Gametes were collected by intracoelomic injection of 0.5 M KCl. Fertilization and embryo culture were carried out according to Adams et al. (2019). Embryos were cultured at 15° C in 4-liter plastic beakers fitted with battery powered stirrers. Embryos were harvested at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr post- fertilization (hpf). The 9 samples were derived from two separate embryo cultures. For the 0 hpf time point, embryos were collected immediately after fertilization. Total RNA (75-100 g/sample) was isolated using the RNeasy Plus Mini kit (Qiagen Cat. No. 74134) and the quality of the preparations was confirmed using an RNA TapeStation (Agilent). RNA integrity numbers (RINs) for all samples were 9.8-10.0. RNA samples were stored at -80°C. CAGE library preparation, sequencing, mapping, and eRNA identification were performed by DNAFORM (Yokohama, Kanagawa, Japan) Illumina HiSeq 2000 gds 305184292 GSM5184292 GEO Accession GSM5184292 SRX10386351 GSM5184293 SRP311353 SRS8509482 GSM5184293 RNA-Seq TRANSCRIPTOMIC CAGE Adult S. purpuratus were obtained from Pat Leahy (Caltech). Gametes were collected by intracoelomic injection of 0.5 M KCl. Fertilization and embryo culture were carried out according to Adams et al. (2019). Embryos were cultured at 15° C in 4-liter plastic beakers fitted with battery powered stirrers. Embryos were harvested at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr post- fertilization (hpf). The 9 samples were derived from two separate embryo cultures. For the 0 hpf time point, embryos were collected immediately after fertilization. Total RNA (75-100 g/sample) was isolated using the RNeasy Plus Mini kit (Qiagen Cat. No. 74134) and the quality of the preparations was confirmed using an RNA TapeStation (Agilent). RNA integrity numbers (RINs) for all samples were 9.8-10.0. RNA samples were stored at -80°C. CAGE library preparation, sequencing, mapping, and eRNA identification were performed by DNAFORM (Yokohama, Kanagawa, Japan) Illumina HiSeq 2000 gds 305184293 GSM5184293 GEO Accession GSM5184293 SRX10386352 GSM5184294 SRP311353 SRS8509483 GSM5184294 RNA-Seq TRANSCRIPTOMIC CAGE Adult S. purpuratus were obtained from Pat Leahy (Caltech). Gametes were collected by intracoelomic injection of 0.5 M KCl. Fertilization and embryo culture were carried out according to Adams et al. (2019). Embryos were cultured at 15° C in 4-liter plastic beakers fitted with battery powered stirrers. Embryos were harvested at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr post- fertilization (hpf). The 9 samples were derived from two separate embryo cultures. For the 0 hpf time point, embryos were collected immediately after fertilization. Total RNA (75-100 g/sample) was isolated using the RNeasy Plus Mini kit (Qiagen Cat. No. 74134) and the quality of the preparations was confirmed using an RNA TapeStation (Agilent). RNA integrity numbers (RINs) for all samples were 9.8-10.0. RNA samples were stored at -80°C. CAGE library preparation, sequencing, mapping, and eRNA identification were performed by DNAFORM (Yokohama, Kanagawa, Japan) Illumina HiSeq 2000 gds 305184294 GSM5184294 GEO Accession GSM5184294 SRX10386353 GSM5184295 SRP311353 SRS8509484 GSM5184295 RNA-Seq TRANSCRIPTOMIC CAGE Adult S. purpuratus were obtained from Pat Leahy (Caltech). Gametes were collected by intracoelomic injection of 0.5 M KCl. Fertilization and embryo culture were carried out according to Adams et al. (2019). Embryos were cultured at 15° C in 4-liter plastic beakers fitted with battery powered stirrers. Embryos were harvested at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr post- fertilization (hpf). The 9 samples were derived from two separate embryo cultures. For the 0 hpf time point, embryos were collected immediately after fertilization. Total RNA (75-100 g/sample) was isolated using the RNeasy Plus Mini kit (Qiagen Cat. No. 74134) and the quality of the preparations was confirmed using an RNA TapeStation (Agilent). RNA integrity numbers (RINs) for all samples were 9.8-10.0. RNA samples were stored at -80°C. CAGE library preparation, sequencing, mapping, and eRNA identification were performed by DNAFORM (Yokohama, Kanagawa, Japan) Illumina HiSeq 2000 gds 305184295 GSM5184295 GEO Accession GSM5184295 SRX10386354 GSM5184287 SRP311353 SRS8509485 GSM5184287 RNA-Seq TRANSCRIPTOMIC CAGE Adult S. purpuratus were obtained from Pat Leahy (Caltech). Gametes were collected by intracoelomic injection of 0.5 M KCl. Fertilization and embryo culture were carried out according to Adams et al. (2019). Embryos were cultured at 15° C in 4-liter plastic beakers fitted with battery powered stirrers. Embryos were harvested at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr post- fertilization (hpf). The 9 samples were derived from two separate embryo cultures. For the 0 hpf time point, embryos were collected immediately after fertilization. Total RNA (75-100 g/sample) was isolated using the RNeasy Plus Mini kit (Qiagen Cat. No. 74134) and the quality of the preparations was confirmed using an RNA TapeStation (Agilent). RNA integrity numbers (RINs) for all samples were 9.8-10.0. RNA samples were stored at -80°C. CAGE library preparation, sequencing, mapping, and eRNA identification were performed by DNAFORM (Yokohama, Kanagawa, Japan) Illumina HiSeq 2000 gds 305184287 GSM5184287 GEO Accession GSM5184287 SRX10386355 GSM5184288 SRP311353 SRS8509486 GSM5184288 RNA-Seq TRANSCRIPTOMIC CAGE Adult S. purpuratus were obtained from Pat Leahy (Caltech). Gametes were collected by intracoelomic injection of 0.5 M KCl. Fertilization and embryo culture were carried out according to Adams et al. (2019). Embryos were cultured at 15° C in 4-liter plastic beakers fitted with battery powered stirrers. Embryos were harvested at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr post- fertilization (hpf). The 9 samples were derived from two separate embryo cultures. For the 0 hpf time point, embryos were collected immediately after fertilization. Total RNA (75-100 g/sample) was isolated using the RNeasy Plus Mini kit (Qiagen Cat. No. 74134) and the quality of the preparations was confirmed using an RNA TapeStation (Agilent). RNA integrity numbers (RINs) for all samples were 9.8-10.0. RNA samples were stored at -80°C. CAGE library preparation, sequencing, mapping, and eRNA identification were performed by DNAFORM (Yokohama, Kanagawa, Japan) Illumina HiSeq 2000 gds 305184288 GSM5184288 GEO Accession GSM5184288