SRX10424647 GSM5203389 SRP311831 SRS8545516 GSM5203389 RNA-Seq TRANSCRIPTOMIC cDNA Articular cartilage obtained from non-arthritic and OA patients was finely minced under sterile conditions and digested with 0.25% Collagenase type II from Clostrudium histolyticum (Sigma-Aldrich) dissolved in DMEM at 37oC overnight. After enzymatic digestion, cells were pelleted by centrifugation, washed, filtered through 50uM cell strainer, and resuspended in 1ml PBS. The scRNA-Seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3' V3 Reagent Kits (10X Genomics, Pleasanton, CA) and sequenced the Illumina HiSeq 4,000 with read length of 150 bp by the NYU Genome Technology Center. Illumina HiSeq 2500 gds 305203389 GSM5203389 GEO Accession GSM5203389 SRX10424648 GSM5203390 SRP311831 SRS8545517 GSM5203390 RNA-Seq TRANSCRIPTOMIC cDNA Articular cartilage obtained from non-arthritic and OA patients was finely minced under sterile conditions and digested with 0.25% Collagenase type II from Clostrudium histolyticum (Sigma-Aldrich) dissolved in DMEM at 37oC overnight. After enzymatic digestion, cells were pelleted by centrifugation, washed, filtered through 50uM cell strainer, and resuspended in 1ml PBS. The scRNA-Seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3' V3 Reagent Kits (10X Genomics, Pleasanton, CA) and sequenced the Illumina HiSeq 4,000 with read length of 150 bp by the NYU Genome Technology Center. Illumina HiSeq 2500 gds 305203390 GSM5203390 GEO Accession GSM5203390 SRX10424649 GSM5203391 SRP311831 SRS8545518 GSM5203391 RNA-Seq TRANSCRIPTOMIC cDNA Articular cartilage obtained from non-arthritic and OA patients was finely minced under sterile conditions and digested with 0.25% Collagenase type II from Clostrudium histolyticum (Sigma-Aldrich) dissolved in DMEM at 37oC overnight. After enzymatic digestion, cells were pelleted by centrifugation, washed, filtered through 50uM cell strainer, and resuspended in 1ml PBS. The scRNA-Seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3' V3 Reagent Kits (10X Genomics, Pleasanton, CA) and sequenced the Illumina HiSeq 4,000 with read length of 150 bp by the NYU Genome Technology Center. Illumina HiSeq 2500 gds 305203391 GSM5203391 GEO Accession GSM5203391 SRX10424650 GSM5203392 SRP311831 SRS8545519 GSM5203392 RNA-Seq TRANSCRIPTOMIC cDNA Articular cartilage obtained from non-arthritic and OA patients was finely minced under sterile conditions and digested with 0.25% Collagenase type II from Clostrudium histolyticum (Sigma-Aldrich) dissolved in DMEM at 37oC overnight. After enzymatic digestion, cells were pelleted by centrifugation, washed, filtered through 50uM cell strainer, and resuspended in 1ml PBS. The scRNA-Seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3' V3 Reagent Kits (10X Genomics, Pleasanton, CA) and sequenced the Illumina HiSeq 4,000 with read length of 150 bp by the NYU Genome Technology Center. Illumina HiSeq 2500 gds 305203392 GSM5203392 GEO Accession GSM5203392 SRX10424651 GSM5203393 SRP311831 SRS8545520 GSM5203393 RNA-Seq TRANSCRIPTOMIC cDNA Articular cartilage obtained from non-arthritic and OA patients was finely minced under sterile conditions and digested with 0.25% Collagenase type II from Clostrudium histolyticum (Sigma-Aldrich) dissolved in DMEM at 37oC overnight. After enzymatic digestion, cells were pelleted by centrifugation, washed, filtered through 50uM cell strainer, and resuspended in 1ml PBS. The scRNA-Seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3' V3 Reagent Kits (10X Genomics, Pleasanton, CA) and sequenced the Illumina HiSeq 4,000 with read length of 150 bp by the NYU Genome Technology Center. Illumina HiSeq 2500 gds 305203393 GSM5203393 GEO Accession GSM5203393 SRX10424652 GSM5203394 SRP311831 SRS8545521 GSM5203394 RNA-Seq TRANSCRIPTOMIC cDNA Articular cartilage obtained from non-arthritic and OA patients was finely minced under sterile conditions and digested with 0.25% Collagenase type II from Clostrudium histolyticum (Sigma-Aldrich) dissolved in DMEM at 37oC overnight. After enzymatic digestion, cells were pelleted by centrifugation, washed, filtered through 50uM cell strainer, and resuspended in 1ml PBS. The scRNA-Seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3' V3 Reagent Kits (10X Genomics, Pleasanton, CA) and sequenced the Illumina HiSeq 4,000 with read length of 150 bp by the NYU Genome Technology Center. Illumina HiSeq 2500 gds 305203394 GSM5203394 GEO Accession GSM5203394 SRX10424653 GSM5203395 SRP311831 SRS8545522 GSM5203395 RNA-Seq TRANSCRIPTOMIC cDNA Articular cartilage obtained from non-arthritic and OA patients was finely minced under sterile conditions and digested with 0.25% Collagenase type II from Clostrudium histolyticum (Sigma-Aldrich) dissolved in DMEM at 37oC overnight. After enzymatic digestion, cells were pelleted by centrifugation, washed, filtered through 50uM cell strainer, and resuspended in 1ml PBS. The scRNA-Seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3' V3 Reagent Kits (10X Genomics, Pleasanton, CA) and sequenced the Illumina HiSeq 4,000 with read length of 150 bp by the NYU Genome Technology Center. Illumina HiSeq 2500 gds 305203395 GSM5203395 GEO Accession GSM5203395