SRX10914001 GSM5320168 SRP320176 SRS9004286 GSM5320168 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320168 GSM5320168 GEO Accession GSM5320168 SRX10914002 GSM5320169 SRP320176 SRS9004287 GSM5320169 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320169 GSM5320169 GEO Accession GSM5320169 SRX10914003 GSM5320170 SRP320176 SRS9004288 GSM5320170 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320170 GSM5320170 GEO Accession GSM5320170 SRX10914004 GSM5320171 SRP320176 SRS9004289 GSM5320171 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320171 GSM5320171 GEO Accession GSM5320171 SRX10914005 GSM5320172 SRP320176 SRS9004290 GSM5320172 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320172 GSM5320172 GEO Accession GSM5320172 SRX10914006 GSM5320173 SRP320176 SRS9004291 GSM5320173 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320173 GSM5320173 GEO Accession GSM5320173 SRX10914007 GSM5320152 SRP320176 SRS9004292 GSM5320152 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320152 GSM5320152 GEO Accession GSM5320152 SRX10914008 GSM5320153 SRP320176 SRS9004293 GSM5320153 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320153 GSM5320153 GEO Accession GSM5320153 SRX10914009 GSM5320154 SRP320176 SRS9004294 GSM5320154 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320154 GSM5320154 GEO Accession GSM5320154 SRX10914010 GSM5320155 SRP320176 SRS9004295 GSM5320155 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320155 GSM5320155 GEO Accession GSM5320155 SRX10914011 GSM5320156 SRP320176 SRS9004296 GSM5320156 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320156 GSM5320156 GEO Accession GSM5320156 SRX10914012 GSM5320157 SRP320176 SRS9004297 GSM5320157 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320157 GSM5320157 GEO Accession GSM5320157 SRX10914013 GSM5320158 SRP320176 SRS9004298 GSM5320158 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320158 GSM5320158 GEO Accession GSM5320158 SRX10914014 GSM5320159 SRP320176 SRS9004299 GSM5320159 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320159 GSM5320159 GEO Accession GSM5320159 SRX10914015 GSM5320160 SRP320176 SRS9004300 GSM5320160 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320160 GSM5320160 GEO Accession GSM5320160 SRX10914016 GSM5320161 SRP320176 SRS9004301 GSM5320161 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320161 GSM5320161 GEO Accession GSM5320161 SRX10914017 GSM5320162 SRP320176 SRS9004302 GSM5320162 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320162 GSM5320162 GEO Accession GSM5320162 SRX10914018 GSM5320163 SRP320176 SRS9004303 GSM5320163 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320163 GSM5320163 GEO Accession GSM5320163 SRX10914019 GSM5320164 SRP320176 SRS9004304 GSM5320164 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320164 GSM5320164 GEO Accession GSM5320164 SRX10914020 GSM5320165 SRP320176 SRS9004305 GSM5320165 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320165 GSM5320165 GEO Accession GSM5320165 SRX10914021 GSM5320166 SRP320176 SRS9004306 GSM5320166 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320166 GSM5320166 GEO Accession GSM5320166 SRX10914022 GSM5320167 SRP320176 SRS9004307 GSM5320167 RNA-Seq TRANSCRIPTOMIC cDNA Human breast surgical samples were mechanically dissociated with scalpels, diged with 2mg/ml collagenease I in DMEM overnight with 20U/ml DNaseI, these created human organoids that were viably cryoperserved. Thawed human organoids were then digested with 0.05% trypsin for 6 mins before they were stained for FACS. Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell. Illumina NovaSeq 6000 gds 305320167 GSM5320167 GEO Accession GSM5320167