SRP321811 PRJNA733507 GSE175768 Spatial alanine metabolism determines local growth dynamics of Escherichia coli colonies By using transcriptomics, we studied the spatiotemporal transcriptional changes in Escherichia coli biofilm colonies Overall design: Whole colony measurenments were performed on colonies grown for 12 h to 72 h in triplicate (samples 1-24). For colonies grown for 72 h, the transcriptional profile of anoxic and oxic cells was analyzed using FACS and RNAseq with 4 replicates (samples 41-48). Samples 25-40 are controls GSE175768 pubmed 34751128