SRP322157 PRJNA734154 Semliki Forest virus, Aedes aegypti derived cells Raw sequence reads Small RNA sequencing profiles to determine the effects on the production and methylation of small RNAs in the presence or lack of Ago2. Therefore, Aedes aegypti-derived AF5 cells or Ago2 knockout cells (AF525) were infected with Semliki Forest virus. To determine the methylation, total RNA was either left untreated or treated by beta-elimination; following small RNA sequencing. Results show that endogenous and exogenous siRNA methylation is dependent on the presence of Ago2.