SRX11312529 GSM5411273 SRP326256 SRS9341543 GSM5411273 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow derived cell were isolated from C57BL/6 mice tibia and femur using an aseptic technique. The bone marrow was flushed out of the bone marrow cavity by using a 26-gauge needle with 10ml PBS. Subsequently cell suspension was passed through a 70µm nylon mesh. After a 10 min centrifugation step at 500 x g, cells were incubated in 2ml red cell lysis buffer for red blood cell removal. After 4 min incubation time, lysis was stopped by adding 23 ml PBS.. Cells were resuspended in PBS and FACS sorted for CD45 cells using an AriaIII-sorter and 7-AAD as indicator for dead cells. The cell suspensions were counted with Moxi cell counter and diluted according to manufacturer's protocol to obtain 10.000 single cell data points per sample. Single cell libraries were generated on 10x Chromium Controler (10x Genomics) with 16000 cells as input following standard protocol. NextSeq 500 gds 305411273 GSM5411273 GEO Accession GSM5411273