SRX11325988 GSM5412578 SRP326471 SRS9359029 GSM5412578 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412578 GSM5412578 GEO Accession GSM5412578 SRX11325989 GSM5412579 SRP326471 SRS9359030 GSM5412579 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412579 GSM5412579 GEO Accession GSM5412579 SRX11325990 GSM5412580 SRP326471 SRS9359031 GSM5412580 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412580 GSM5412580 GEO Accession GSM5412580 SRX11325991 GSM5412581 SRP326471 SRS9359032 GSM5412581 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412581 GSM5412581 GEO Accession GSM5412581 SRX11325992 GSM5412582 SRP326471 SRS9359033 GSM5412582 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412582 GSM5412582 GEO Accession GSM5412582 SRX11325993 GSM5412583 SRP326471 SRS9359034 GSM5412583 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412583 GSM5412583 GEO Accession GSM5412583 SRX11325994 GSM5412584 SRP326471 SRS9359035 GSM5412584 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412584 GSM5412584 GEO Accession GSM5412584 SRX11325995 GSM5412585 SRP326471 SRS9359036 GSM5412585 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412585 GSM5412585 GEO Accession GSM5412585 SRX11325996 GSM5412586 SRP326471 SRS9359037 GSM5412586 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412586 GSM5412586 GEO Accession GSM5412586 SRX11325997 GSM5412587 SRP326471 SRS9359038 GSM5412587 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412587 GSM5412587 GEO Accession GSM5412587 SRX11325998 GSM5412588 SRP326471 SRS9359039 GSM5412588 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412588 GSM5412588 GEO Accession GSM5412588 SRX11325999 GSM5412589 SRP326471 SRS9359040 GSM5412589 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412589 GSM5412589 GEO Accession GSM5412589 SRX11326000 GSM5412590 SRP326471 SRS9359041 GSM5412590 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412590 GSM5412590 GEO Accession GSM5412590 SRX11326001 GSM5412591 SRP326471 SRS9359042 GSM5412591 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412591 GSM5412591 GEO Accession GSM5412591 SRX11326002 GSM5412592 SRP326471 SRS9359043 GSM5412592 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412592 GSM5412592 GEO Accession GSM5412592 SRX11326003 GSM5412593 SRP326471 SRS9359044 GSM5412593 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412593 GSM5412593 GEO Accession GSM5412593 SRX11326004 GSM5412594 SRP326471 SRS9359045 GSM5412594 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412594 GSM5412594 GEO Accession GSM5412594 SRX11326005 GSM5412595 SRP326471 SRS9359046 GSM5412595 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412595 GSM5412595 GEO Accession GSM5412595 SRX11326006 GSM5412596 SRP326471 SRS9359047 GSM5412596 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412596 GSM5412596 GEO Accession GSM5412596 SRX11326007 GSM5412597 SRP326471 SRS9359048 GSM5412597 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412597 GSM5412597 GEO Accession GSM5412597 SRX11326008 GSM5412598 SRP326471 SRS9359049 GSM5412598 RNA-Seq TRANSCRIPTOMIC cDNA Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Together with fixed OD units of S. pombe cells as spike in, total RNA was extracted using the hot acid phenol protocol (acid phenol:chloroform:isoamyl alcohol 125:24:1) and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in DEPC-treated sterile water. Total RNA from yeast was incubated with rDNase (Machery-Nagel rDNase Set) and column purified (Machery-Nagel NucleoSpin RNA) prior to sequencing library preparation. Total RNA was used to generate strand-specific libraries for polyadenylated RNA sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina RS-122-2101) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2200 TapeStation with D1000 screentape (Agilent Technologies). Each library was sequenced on the HiSeq 2500 platform using V4 chemistry (Illumina) and typically generated ~55 million 101 bp strand-specific paired-end reads per sample. Illumina HiSeq 4000 gds 305412598 GSM5412598 GEO Accession GSM5412598